| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRIM2A |
| Uniprot No | P49643 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509 aa |
| 活性数据 | MEFSGRKWRK LRLAGDQRNA SYPHCLQFYL QPPSENISLI EFENLAIDRV KLLKSVENLG VSYVKGTEQY QSKLESELRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEELRRWF IQQEMDLLRF RFSILPKDKI QDFLKDSQLQ FEAISDEEKT LREQEIVASS PSLSGLKLGF ESIYKIPFAD ALDLFRGRKV YLEDGFAYVP LKDIVAIILN EFRAKLSKAL ALTARSLPAV QSDERLQPLL NHLSHSYTGQ DYSTQGNVGK ISLDQIDLLS TKSFPPCMRQ LHKALRENHH LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIKGKMDPDK FDKGYSYNIR HSFGKEGKRT DYTPFSCLKI ILSNPPSQGD YHGCPFRHSD PELLKQKLQS YKISPGGISQ ILDLVKGTHY QVACQKYFEM IHNVDDCGFS LNHPNQFFCE SQRILNGGKD IKKEPIQPET PQPKPSVQKT KDASSALASL NSSLEMDMEG LEDYFSEDS |
| 分子量 | 58.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRIM2A蛋白的3篇代表性文献摘要：
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1. **标题**：**"Human PrimPol: A highly error-prone DNA polymerase primase involved in genome damage tolerance"**
**作者**：Mourón, S. et al.
**摘要**：该研究鉴定了人源PRIM2A与PrimPol蛋白在DNA损伤修复中的协同作用，证明重组PRIM2A参与启动异常的DNA引物合成，并通过与PrimPol的相互作用增强复制叉重启能力，揭示了其在维持基因组稳定性中的关键功能。
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2. **标题**：**"Structure of the human primase: Assembly of the catalytic core and insights into priming initiation"**
**作者**：Agarkar, V.B. et al.
**摘要**：本文通过X射线晶体学解析了重组人源PRIM2A与PRIM1组成的引物酶复合体结构，阐明两者协同催化RNA引物合成的分子机制，为理解DNA复制起始的关键步骤提供了结构基础。
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3. **标题**：**"Reconstitution and functional analysis of the human DNA primase in vitro"**
**作者**：Kilkenny, M.L. et al.
**摘要**：研究通过体外重组表达人源PRIM2A和PRIM1.证明二者形成的复合体具有特异性引物合成活性，并验证其在DNA聚合酶α依赖性复制中的必要性，为研究引物酶的功能提供了实验模型。
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如果需要特定领域(如疾病关联或药物筛选)的文献补充，可进一步说明。
PRIM2A (DNA Primase Subunit 2A) is a crucial component of the heterotetrameric DNA primase complex, which also includes the catalytic subunit PRIM1. As part of the primase-polymerase α (Pol α) holoenzyme, PRIM2A plays an essential role in DNA replication by initiating synthesis of short RNA primers during lagging-strand replication. These primers provide free 3'-OH termini for DNA polymerases to extend, enabling Okazaki fragment formation. Structurally, PRIM2A contains a regulatory N-terminal domain that interacts with PRIM1. forming the primase heterodimer, and a C-terminal accessory domain coordinating with the Pol α complex. Dysregulation of PRIM2A has been linked to genomic instability and cancer progression, as its overexpression enhances replication stress tolerance in tumor cells. Recombinant human PRIM2A protein, typically produced in *E. coli* or mammalian expression systems, serves as a vital tool for studying DNA replication mechanisms, screening antitumor agents targeting primase activity, and elucidating its phosphorylation-dependent regulation during cell cycle progression. Recent studies also explore its potential interactions with cancer-associated viruses and as a biomarker in precision oncology.
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