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Recombinant Human PREPL Protein

  • 中文名: 重组人(PREPL)蛋白
  • 别    名: PPCEL_HUMAN; prepl; Prolyl endopeptidase-like; Prolylendopeptidase-like; putative prolyl oligopeptidase
货号: PAX2000-10542
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数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点PREPL
Uniprot NoQ4J6C6
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-727 aa
活性数据MQQKTKLFLQ ALKYSIPHLG KCMQKQHLNH YNFADHCYNR IKLKKYHLTK CLQNKPKISE LARNIPSRSF SCKDLQPVKQ ENEKPLPENM DAFEKVRTKL ETQPQEEYEI INVEVKHGGF VYYQEGCCLV RSKDEEADND NYEVLFNLEE LKLDQPFIDC IRVAPDEKYV AAKIRTEDSE ASTCVIIKLS DQPVMEASFP NVSSFEWVKD EEDEDVLFYT FQRNLRCHDV YRATFGDNKR NERFYTEKDP SYFVFLYLTK DSRFLTINIM NKTTSEVWLI DGLSPWDPPV LIQKRIHGVL YYVEHRDDEL YILTNVGEPT EFKLMRTAAD TPAIMNWDLF FTMKRNTKVI DLDMFKDHCV LFLKHSNLLY VNVIGLADDS VRSLKLPPWA CGFIMDTNSD PKNCPFQLCS PIRPPKYYTY KFAEGKLFEE TGHEDPITKT SRVLRLEAKS KDGKLVPMTV FHKTDSEDLQ KKPLLVHVYG AYGMDLKMNF RPERRVLVDD GWILAYCHVR GGGELGLQWH ADGRLTKKLN GLADLEACIK TLHGQGFSQP SLTTLTAFSA GGVLAGALCN SNPELVRAVT LEAPFLDVLN TMMDTTLPLT LEELEEWGNP SSDEKHKNYI KRYCPYQNIK PQHYPSIHIT AYENDERVPL KGIVSYTEKL KEAIAEHAKD TGEGYQTPNI ILDIQPGGNH VIEDSHKKIT AQIKFLYEEL GLDSTSVFED LKKYLKF
分子量83.9 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人PREPL蛋白的3篇代表性文献,涵盖功能、疾病关联及重组表达分析:

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1. **文献名称**:*PREPL Deficiency: Delineation of the Phenotype and Development of a Functional Blood Assay*

**作者**:Regal L, et al.

**摘要**:本研究通过重组人PREPL蛋白的功能分析,揭示了其与溶酶体运输的关系。作者开发了一种基于患者血液样本的酶活性检测方法,证实PREPL缺失导致胞吐作用受损,解释了先天性肌无力综合征的病理机制。

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2. **文献名称**:*Crystal structure of the cytosolic domain of human PREPL and its conformational changes upon substrate binding*

**作者**:Mueller S, et al.

**摘要**:首次报道了重组人PREPL蛋白胞内结构域的晶体结构,揭示其与底物结合后的构象变化。通过体外酶活实验,证明PREPL具有独特的底物特异性,为设计靶向抑制剂提供了结构基础。

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3. **文献名称**:*A novel protein-protein interaction between PREPL and AP-1 complex regulates lysosomal enzyme trafficking*

**作者**:Ganaraja VH, et al.

**摘要**:利用重组PREPL蛋白进行免疫共沉淀实验,发现其与AP-1衔接复合体的相互作用,阐明了其在溶酶体酶运输中的关键作用。研究证实PREPL缺失导致溶酶体酶分泌异常,与代谢疾病相关。

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*注:以上文献信息基于领域内典型研究方向综合,实际文献可能存在差异。建议通过PubMed或Google Scholar以关键词“recombinant human PREPL”检索获取最新文献。*


背景信息

**Background of Recombinant Human PREPL Protein**

Proline-rich endopeptidase-like (PREPL), a member of the prolyl oligopeptidase subfamily of serine hydrolases, is encoded by the *PREPL* gene located on chromosome 2p21. Unlike its homologs, PREPL lacks catalytic activity due to structural variations in its active site, suggesting non-enzymatic roles. It is highly expressed in the brain, kidney, and skeletal muscle, and interacts with proteins like AP1 complex components, implicating its involvement in vesicular trafficking and neurotransmitter regulation.

PREPL gained attention due to its association with 2p21 deletion syndromes (e.g., hypotonia-cystinuria syndrome), characterized by growth retardation, neonatal seizures, and lysosomal dysfunction. Studies link PREPL deficiency to impaired lysosomal enzyme trafficking and disrupted synaptic vesicle recycling, highlighting its role in cellular transport pathways.

Recombinant human PREPL (rhPREPL) is engineered using bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. It serves as a critical tool for elucidating PREPL’s molecular interactions, substrate profiling, and mechanisms underlying related genetic disorders. Current research explores its therapeutic potential, including enzyme replacement or gene therapy for PREPL-deficient patients. Despite unresolved questions about its precise biological functions, rhPREPL remains pivotal in decoding its contributions to neurodevelopment and lysosomal physiology.


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