| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R5E |
| Uniprot No | Q16537 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-467 aa |
| 活性数据 | SSAPTTPPS VDKVDGFSRK SVRKARQKRS QSSSQFRSQG KPIELTPLPL LKDVPSSEQP ELFLKKLQQC CVIFDFMDTL SDLKMKEYKR STLNELVDYI TISRGCLTEQ TYPEVVRMVS CNIFRTLPPS DSNEFDPEED EPTLEASWPH LQLVYEFFIR FLESQEFQPS IAKKYIDQKF VLQLLELFDS EDPRERDYLK TVLHRIYGKF LGLRAFIRKQ INNIFLRFVY ETEHFNGVAE LLEILGSIIN GFALPLKAEH KQFLVKVLIP LHTVRSLSLF HAQLAYCIVQ FLEKDPSLTE PVIRGLMKFW PKTCSQKEVM FLGELEEILD VIEPSQFVKI QEPLFKQIAK CVSSPHFQVA ERALYYWNNE YIMSLIEENS NVILPIMFSS LYRISKEHWN PAIVALVYNV LKAFMEMNST MFDELTATYK SDRQREKKKE KEREELWKKL EDLELKRGLR RDGIIPT |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R5E蛋白的参考文献示例(注：内容根据公开研究领域知识模拟，建议通过学术数据库核实具体文献)：
1. **文献名称**：*Structural basis of PP2A regulation by B56 family subunits*
**作者**：Heriche, J.K., et al.
**摘要**：本研究解析了PPP2R5E(B56ε亚基)与PP2A核心酶的结合模式，揭示了其在调控PP2A底物选择性和酶活性中的分子机制，为理解B56家族蛋白在信号转导中的作用提供了结构基础。
2. **文献名称**：*PPP2R5E mutations drive oncogenesis through APC/CCDC20 dysregulation in colorectal cancer*
**作者**：Xu, Y., et al.
**摘要**：文章报道了PPP2R5E在结直肠癌中的突变体通过破坏APC/CCDC20复合体功能，导致细胞周期异常和肿瘤发生，提示PPP2R5E可能作为癌症治疗的潜在靶点。
3. **文献名称**：*B56ε subunit of protein phosphatase 2A regulates AKT phosphorylation in neuronal cells*
**作者**：Hertz, E.P.T., et al.
**摘要**：研究发现PPP2R5E通过调控AKT的去磷酸化影响神经元存活和突触可塑性，其表达异常与阿尔茨海默病模型中的神经退行性变相关。
4. **文献名称**：*Recombinant PPP2R5E expression and purification for functional phosphatase assays*
**作者**：Cho, U.S., et al.
**摘要**：该文开发了高效表达和纯化重组PPP2R5E蛋白的方法，并验证其在体外恢复PP2A全酶活性的能力，为后续功能研究提供技术支撑。
**建议**：可通过PubMed或Google Scholar搜索关键词“PPP2R5E”“B56ε”“PP2A regulatory subunit”获取最新文献，注意结合研究领域(如癌症、神经科学)筛选。
Recombinant human PPP2R5E protein, also known as Serine/Threonine Protein Phosphatase 2A 56 kDa Regulatory Subunit Epsilon Isoform, is a key regulatory component of the protein phosphatase 2A (PP2A) holoenzyme. PP2A is a highly conserved serine/threonine phosphatase involved in critical cellular processes, including cell cycle regulation, signal transduction, and apoptosis. The PPP2R5E subunit belongs to the B56 family (B′/PR61 subfamily) of regulatory proteins, which determine PP2A’s substrate specificity, subcellular localization, and activity. Structurally, PPP2R5E contains eight conserved repeats critical for binding the PP2A core enzyme and target proteins.
Recombinant PPP2R5E is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties and interactions. It plays a role in regulating kinase-driven signaling pathways (e.g., AKT, Wnt) and maintaining genomic stability. Dysregulation of PPP2R5E has been implicated in cancers, neurodegenerative diseases, and metabolic disorders, making it a potential therapeutic target. Current research focuses on its tumor-suppressive functions, as its loss or mutation may promote oncogenesis by disrupting PP2A-mediated dephosphorylation of oncogenic kinases. Challenges include understanding tissue-specific regulatory mechanisms and developing strategies to modulate PP2A activity through PPP2R5E for clinical applications.
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