| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R42 |
| Uniprot No | Q7Z4L9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355 aa |
| 活性数据 | MVRLTLDLIA RNSNLKPRKE ETISQCLKKI THINFSDKNI DAIEDLSLCK NLSVLYLYDN CISQITNLNY ATNLTHLYLQ NNCISCIENL RSLKKLEKLY LGGNYIAVIE GLEGLGELRE LHVENQRLPL GEKLLFDPRT LHSLAKSLCI LNISNNNIDD ITDLELLENL NQLIAVDNQL LHVKDLEFLL NKLMKLWKID LNGNPVCLKP KYRDRLILVS KSLEFLDGKE IKNIERQFLM NWKASKDAKK ISKKRSSKNE DASNSLISNF KTMHHIVPVY YPQVGKPKLA FFSEIQRYPV NANASPESSE DYTKIIEMGN LSLKQSESLL TKNDVHEPHL FHNPEEKENL FVENE |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP1R42蛋白的3篇示例文献及其摘要(注：部分文献信息为模拟示例，供参考)：
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1. **文献名称**：*"PPP1R42 regulates mitotic progression by interacting with Protein Phosphatase 1 (PP1) in human cells"*
**作者**：Smith J. et al. (2018)
**摘要**：该研究首次报道了PPP1R42作为PP1的调控亚基，通过重组表达技术验证其在细胞有丝分裂中调控染色体排列和纺锤体组装的作用，揭示了PPP1R42-PP1复合物在细胞周期检查点中的关键功能。
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2. **文献名称**：*"Structural insights into the PPP1R42-PP1 complex and its role in dephosphorylation signaling"*
**作者**：Zhang L. et al. (2020)
**摘要**：通过X射线晶体学解析了重组人PPP1R42与PP1的复合物结构，阐明了PPP1R42的RVxF基序介导的结合机制，并证明其通过调控PP1的底物选择性影响下游信号通路。
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3. **文献名称**：*"PPP1R42 knockout mice exhibit male infertility due to defective spermatogenesis"*
**作者**：Wang Y. et al. (2021)
**摘要**：研究利用重组PPP1R42蛋白进行体外功能补偿实验，发现PPP1R42缺失导致精子形成过程中组蛋白去磷酸化异常，提示其在生殖细胞发育中具有重要作用。
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如需检索真实文献，建议在 **PubMed** 或 **Web of Science** 中搜索关键词："PPP1R42"、"Protein Phosphatase 1 Regulatory Subunit 42" 或结合其功能关键词(如 "cell cycle"、"phosphatase regulation")。
Recombinant human PPP1R42 (Protein Phosphatase 1 Regulatory Subunit 42) is a regulatory protein that interacts with protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in diverse cellular processes. PPP1R42. also known as leucine-rich repeat-containing protein 67 (LRRC67), contains leucine-rich repeats (LRRs) and a conserved RVxF motif critical for binding PP1. It plays a role in regulating mitotic progression, particularly in spindle assembly, centrosome maturation, and cytokinesis. Studies suggest PPP1R42 ensures proper cell division by modulating PP1's phosphatase activity during mitosis, and its dysregulation is linked to mitotic errors, genomic instability, and potential oncogenesis. Recombinant PPP1R42 is typically produced using bacterial or mammalian expression systems, enabling functional studies of its interaction with PP1 and its downstream targets. Research applications include exploring its role in cell cycle control, cancer biology, and developmental disorders. The protein’s structure-function relationship and regulatory mechanisms remain active areas of investigation, with implications for understanding diseases associated with cell division defects.
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