| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPM1J |
| Uniprot No | Q5JR12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505 aa |
| 活性数据 | MLNRVRSAVA HLVSSGGAPP PRPKSPDLPN AASAPPAAAP EAPRSPPAKA GSGSATPAKA VEARASFSRP TFLQLSPGGL RRADDHAGRA VQSPPDTGRR LPWSTGYAEV INAGKSRHNE DQACCEVVYV EGRRSVTGVP REPSRGQGLC FYYWGLFDGH AGGGAAEMAS RLLHRHIREQ LKDLVEILQD PSPPPLCLPT TPGTPDSSDP SHLLGPQSCW SSQKEVSHES LVVGAVENAF QLMDEQMARE RRGHQVEGGC CALVVIYLLG KVYVANAGDS RAIIVRNGEI IPMSREFTPE TERQRLQLLG FLKPELLGSE FTHLEFPRRV LPKELGQRML YRDQNMTGWA YKKIELEDLR FPLVCGEGKK ARVMATIGVT RGLGDHSLKV CSSTLPIKPF LSCFPEVRVY DLTQYEHCPD DVLVLGTDGL WDVTTDCEVA ATVDRVLSAY EPNDHSRYTA LAQALVLGAR GTPRDRGWRL PNNKLGSGDD ISVFVIPLGG PGSYS |
| 分子量 | 54.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的示例参考文献，供参考。由于PPM1J的研究相对较少，建议通过PubMed或Google Scholar等平台获取最新文献：
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1. **Title**: *Molecular Characterization of PPM1J as a Novel Protein Phosphatase in Human Tissues*
**Authors**: Zhang L, et al.
**Summary**: 本研究首次鉴定了人PPM1J蛋白的磷酸酶活性，发现其依赖Mg²⁺/Mn²⁺特异性去磷酸化MAPK信号通路中的关键蛋白，提示其在细胞应激反应中的调控作用。
2. **Title**: *PPM1J Regulates Hepatic Lipid Metabolism through AMPK Signaling*
**Authors**: Tanaka K, et al.
**Summary**: 发现PPM1J通过去磷酸化激活AMPK，调控肝脏脂代谢稳态。动物实验表明PPM1J缺失会加重高脂饮食诱导的小鼠脂肪肝病变。
3. **Title**: *Genomic Localization and Expression Pattern of PPM1J in Cancer Cell Lines*
**Authors**: Gupta R, et al.
**Summary**: 通过癌症细胞系筛选，揭示了PPM1J基因位于19号染色体(19q13.2)，并发现其在乳腺癌细胞中低表达，可能与ERK信号异常激活相关。
4. **Title**: *Structural Insights into PPM1J Phosphatase Domain and Substrate Specificity*
**Authors**: Müller S, et al.
**Summary**: 利用X射线晶体学解析了PPM1J催化结构域的三维结构，揭示了其底物结合口袋的独特特征，为设计靶向抑制剂提供了结构基础。
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**注意**：上述内容为模拟示例，非真实文献。建议访问以下资源查找真实文献：
- **PubMed**: https://pubmed.ncbi.nlm.nih.gov
- **Google Scholar**: https://scholar.google.com
(可尝试搜索关键词：*PPM1J phosphatase* / *PPM1J function* / *PPM1J structure*)
**Recombinant human PPM1J protein** (Protein Phosphatase, Mg²⁺/Mn²⁺ Dependent 1J), also known as PP2Cζ, is a member of the metal-dependent protein phosphatase PPM family. It plays a role in cellular signaling pathways by dephosphorylating target proteins, thereby regulating diverse biological processes such as metabolism, stress responses, and cell cycle progression. Structurally, PPM1J contains a conserved catalytic domain characteristic of PP2C phosphatases, requiring Mg²⁺ or Mn²⁺ ions for enzymatic activity. Its expression is tissue-specific, with higher levels observed in metabolic tissues like the liver and adipose tissue, suggesting involvement in energy homeostasis.
Research indicates PPM1J interacts with AMP-activated protein kinase (AMPK), a critical regulator of cellular energy balance, potentially modulating AMPK activity in response to metabolic stress. Dysregulation of PPM1J has been implicated in metabolic disorders, including obesity and insulin resistance. Recombinant PPM1J protein is produced using expression systems like *E. coli* or mammalian cells, enabling biochemical studies to elucidate its substrate specificity, regulatory mechanisms, and physiological roles. Recent studies also explore its potential as a therapeutic target for metabolic diseases. However, its full spectrum of functions and downstream effectors remain under investigation, highlighting the need for further research to uncover its pathophysiological relevance and clinical applications.
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