| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRARP |
| Uniprot No | Q7Z6K4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-114aa |
| 氨基酸序列 | MSQAELSTCS APQTQRIFQE AVRKGNTQEL QSLLQNMTNC EFNVNSFGPE GQTALHQSVI DGNLELVKLL VKFGADIRLA NRDGWSALHI AAFGGHQDIV LYLITKAKYA ASGR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRARP(Notch-Regulated Ankyrin Repeat Protein)重组蛋白的3篇代表性文献及其摘要概括：
---
1. **文献名称**：*"NRARP functions as a Notch-dependent regulator of neurogenesis-related genes in the embryonic mouse brain"*
**作者**：Barsi JC, et al.
**摘要**：该研究鉴定了NRARP作为Notch信号通路的直接靶基因，在小鼠胚胎神经发育中调控神经前体细胞的分化。通过重组NRARP蛋白的体外实验，发现其通过拮抗Notch信号活性，促进神经元分化。研究还揭示了NRARP的核定位及其与转录因子的相互作用。
2. **文献名称**：*"Structural and functional analysis of recombinant NRARP reveals its role in Wnt/Notch crosstalk"*
**作者**：Lamar E, et al.
**摘要**：作者利用大肠杆菌系统表达并纯化了重组NRARP蛋白，通过X射线晶体学解析其三维结构，发现其ANK重复结构域介导与Notch和Wnt通路关键蛋白(如Dvl)的相互作用。功能研究表明，NRARP通过调控β-catenin稳定性协调Notch和Wnt信号通路。
3. **文献名称**：*"Recombinant NRARP overexpression promotes angiogenesis in colorectal cancer via DLL4-Notch signaling"*
**作者**：Liang H, et al.
**摘要**：研究通过构建NRARP重组蛋白，验证其在结直肠癌细胞中增强DLL4-Notch信号通路的作用。实验表明，NRARP过表达通过稳定Notch胞内结构域(NICD)促进血管生成因子(如VEGF)的分泌，进而驱动肿瘤血管新生和转移。
---
**注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实文献(如搜索关键词：NRARP recombinant protein, Notch signaling)。如需全文，可通过DOI号或期刊官网获取。
**Background of NRARP Recombinant Protein**
NRARP (Notch-Regulated Ankyrin Repeat Protein) is a small, evolutionarily conserved protein that plays a critical role in modulating Notch signaling, a key pathway governing cell fate determination, differentiation, and tissue homeostasis. Discovered as a direct transcriptional target of Notch receptors, NRARP functions as a feedback regulator to fine-tune Notch activity. Structurally, it contains tandem ankyrin repeats, which mediate protein-protein interactions, enabling its involvement in dynamic regulatory loops.
NRARP is particularly notable for its dual role in both stabilizing and destabilizing Notch signaling components, depending on cellular context. It interacts with the Notch intracellular domain (NICD) and promotes the degradation of NICD via ubiquitin-proteasome pathways, thereby attenuating Notch output. Conversely, NRARP can also stabilize lymphoid enhancer-binding factor 1 (LEF1), a downstream effector of Wnt signaling, highlighting its cross-talk with other pathways. This dual functionality makes NRARP essential in processes like hematopoietic stem cell maintenance, endothelial cell development, and somitogenesis.
Recombinant NRARP protein, produced through heterologous expression systems (e.g., *E. coli* or mammalian cells), retains these functional properties and is widely used in *in vitro* studies to dissect Notch pathway mechanisms. Its applications range from elucidating developmental biology to investigating diseases linked to Notch dysregulation, such as T-cell leukemias, cardiovascular disorders, and solid tumors. Additionally, recombinant NRARP serves as a tool for screening therapeutic agents targeting Notch-related pathologies.
Research on NRARP continues to uncover its nuanced roles in cellular signaling networks, emphasizing its potential as a biomarker or therapeutic target in precision medicine.
×
