| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPEF1 |
| Uniprot No | O14829 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653 aa |
| 活性数据 | MGCSSSSTKT RRSDTSLRAA LIIQNWYRGY KARLKARQHY ALTIFQSIEY ADEQGQMQLS TFFSFMLENY THIHKEELEL RNQSLESEQD MRDRWDYVDS IDVPDSYNGP RLQFPLTCTD IDLLLEAFKE QQILHAHYVL EVLFETKKVL KQMPNFTHIQ TSPSKEVTIC GDLHGKLDDL FLIFYKNGLP SERNPYVFNG DFVDRGKNSI EILMILCVSF LVYPNDLHLN RGNHEDFMMN LRYGFTKEIL HKYKLHGKRI LQILEEFYAW LPIGTIVDNE ILVIHGGISE TTDLNLLHRV ERNKMKSVLI PPTETNRDHD TDSKHNKVGV TFNAHGRIKT NGSPTEHLTE HEWEQIIDIL WSDPRGKNGC FPNTCRGGGC YFGPDVTSKI LNKYQLKMLI RSHECKPEGY EICHDGKVVT IFSASNYYEE GSNRGAYIKL CSGTTPRFFQ YQVTKATCFQ PLRQRVDTME NSAIKILRER VISRKSDLTR AFQLQDHRKS GKLSVSQWAF CMENILGLNL PWRSLSSNLV NIDQNGNVEY MSSFQNIRIE KPVQEAHSTL VETLYRYRSD LEIIFNAIDT DHSGLISVEE FRAMWKLFSS HYNVHIDDSQ VNKLANIMDL NKDGSIDFNE FLKAFYVVHR YEDLMKPDVT NLG |
| 分子量 | 75.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPEF1蛋白的3-4篇代表性文献概述：
1. **文献名称**：*"Characterization of PPEF1. a novel protein phosphatase with homology to Drosophila retinal degeneration C (rdgC)"*
**作者**：Ramulu, P. et al. (2001)
**摘要**：本研究首次报道人源PPEF1蛋白的克隆和功能分析，证明其属于钙离子依赖的丝氨酸/苏氨酸磷酸酶家族，在视网膜中高表达，推测参与光信号传导通路的调控。
2. **文献名称**：*"Expression and functional analysis of recombinant human PPEF1: A potential modulator of tau phosphorylation in Alzheimer's disease"*
**作者**：Smith, A.D. et al. (2005)
**摘要**：通过大肠杆菌系统成功表达重组PPEF1蛋白，体外实验显示其具有去磷酸化阿尔茨海默病相关tau蛋白的活性，提示PPEF1可能在神经退行性疾病中起重要作用。
3. **文献名称**：*"The PPEF1 phosphatase interacts with N-terminal domain of rhodopsin and regulates its dephosphorylation"*
**作者**：Kutty, R.K. et al. (1997)
**摘要**：利用重组PPEF1蛋白进行互作研究，发现其特异性结合视紫红质N端结构域，并调控该蛋白的磷酸化状态，为理解视觉信号终止机制提供新方向。
4. **文献名称**：*"Structural insights into the EF-hand domain-mediated calcium sensitivity of PPEF1 phosphatase"*
**作者**：Li, Y. et al. (2010)
**摘要**：通过晶体学解析重组PPEF1蛋白的EF-hand结构域，揭示钙离子结合对其酶活性的变构调控机制，深化了对该类磷酸酶功能调控的理解。
注：以上内容为模拟文献摘要，实际研究中建议通过PubMed或Web of Science以"PPEF1"或"Protein Phosphatase EF-hand domain 1"为关键词检索最新论文。
**Background of Recombinant Human PPEF1 Protein**
The Protein Phosphatase EF-Hand Domain-Containing 1 (PPEF1) is a serine/threonine phosphatase belonging to the PPP family, distinguished by its N-terminal EF-hand calcium-binding motifs. Predominantly expressed in retinal photoreceptors and sensory neurons, PPEF1 is implicated in regulating calcium-mediated signaling pathways, critical for phototransduction and neuronal function. Its homology with Drosophila retinal degeneration protein (rdgC) suggests a role in maintaining photoreceptor viability, with mutations linked to inherited retinal diseases like retinitis pigmentosa.
Recombinant human PPEF1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Purified PPEF1 retains phosphatase activity, allowing investigation of its enzymatic regulation, substrate specificity, and interaction with calcium or other cellular partners. Research focuses on deciphering its role in retinal homeostasis, neurodegeneration, and potential therapeutic applications. Challenges include elucidating precise physiological targets and mechanisms underlying its disease associations. Current studies leverage recombinant PPEF1 to explore its structure-function relationships and develop targeted interventions for vision-related disorders.
(*Note: Word count: ~250*)
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