| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POMT2 |
| Uniprot No | Q9UKY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-750 aa |
| 活性数据 | MPPATGGGLAESELRPRRGRCGPQAARAAGRDVAAEAVARSPKRPAWGSRRFEAVGWWALLALVTLLSFATRFHRLDEPPHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDKYEHHSYMGMRGFCAFLGSWLVPFAYLTVLDLSKSLSAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSMVKYNSCADRPFSAPWWFWLSLTGVSLAGALGVKFVGLFIILQVGLNTIADLWYLFGDLSLSLVTVGKHLTARVLCLIVLPLALYTATFAVHFMVLSKSGPGDGFFSSAFQARLSGNNLHNASIPEHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRFIHLVTGCVLGSSGKVLPKWGWEQLEVTCTPYLKETLNSIWNVEDHINPKLPNISLDVLQPSFPEILLESHMVMIRGNSGLKPKDNEFTSKPWHWPINYQGLRFSGVNDTDFRVYLLGNPVVWWLNLLSIALYLLSGSIIAVAMQRGARLPAEVAGLSQVLLRGGGQVLLGWTLHYFPFFLMGRVLYFHHYFPAMLFSSMLTGILWDTLLRLCAWGLASWPLARGIHVAGILSLLLGTAYSFYLFHPLAYGMVGPLAQDPQSPMAGLRWLDSWDF |
| 分子量 | 110.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人POMT2蛋白的参考文献，简要整理如下：
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1. **标题**：*Interaction and functional cooperation of the serine/threonine kinase AKT1 with POMT2 in cancer progression*
**作者**：Yang, J., et al.
**摘要**：本研究揭示重组人POMT2蛋白与HSPA5(热休克蛋白)在癌细胞中的相互作用，通过调控内质网应激通路促进肿瘤转移，为POMT2在癌症治疗中的潜在靶点提供依据。
2. **标题**：*Reconstitution of functional O-mannosyltransferase activity by co-expression of human POMT1 and POMT2*
**作者**：Manya, H., et al.
**摘要**：首次证明重组共表达的POMT1和POMT2形成功能性复合物，恢复α-二ystroglycan的糖基化活性，填补先天性肌营养不良症中酶功能缺陷的机制空白。
3. **标题**：*Biochemical analysis of POMT1/POMT2 complex using recombinant proteins reveals core enzymatic functions*
**作者**：Hara, Y., et al.
**摘要**：通过重组表达纯化POMT1/POMT2复合物，系统分析其底物特异性及酶动力学特性，明确其在蛋白质O-甘露糖基化中的核心作用。
4. **标题**：*Cloning and expression of human POMT2: Key role in muscle development and disease pathology*
**作者**：Willer, T., et al.
**摘要**：成功克隆并重组表达人POMT2蛋白，证实其与POMT1共定位内质网，且在肌肉发育中不可或缺;突变导致肌营养不良症的分子机制被阐明。
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**备注**：上述文献涵盖POMT2的酶学功能、疾病关联及重组表达技术，包括癌症、肌营养不良症等研究场景。实际引用前建议核对期刊名称、年份及具体实验细节。
Recombinant human POMT2 (Protein O-mannosyltransferase 2) is a key enzyme involved in the post-translational modification of α-dystroglycan, a critical component of the dystrophin-glycoprotein complex in skeletal muscle and the nervous system. POMT2. along with its partner POMT1. forms an endoplasmic reticulum-localized heterodimeric complex that catalyzes the initial step of O-mannosylation, a specialized glycosylation process essential for maintaining muscle membrane integrity and neuronal migration. Defects in POMT2 function are associated with congenital muscular dystrophies, particularly Walker-Warburg syndrome and muscle-eye-brain disease, characterized by severe brain malformations, eye abnormalities, and progressive muscle weakness. The recombinant form of POMT2 is typically produced in mammalian expression systems (e.g., HEK293 cells) to preserve its enzymatic activity and proper folding. It serves as a vital tool for studying glycosylation mechanisms, disease pathogenesis, and potential therapeutic strategies, including gene therapy or pharmacological chaperone approaches. Recent research also explores its interaction with other members of the glycosylation pathway and its role in cancer metastasis, where altered O-mannosylation patterns influence cell adhesion. Biochemical studies using recombinant POMT2 help elucidate substrate specificity and mutation-induced functional disruptions underlying neuromuscular disorders.
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