| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2L |
| Uniprot No | P62875 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-67 aa |
| 活性数据 | MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK |
| 分子量 | 7.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLR2L蛋白的3篇参考文献及其核心内容概括：
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1. **文献名称**：*Crystal structure of human RNA polymerase II subunit POLR2L*
**作者**：J. Cramer, D.A. Bushnell, R.D. Kornberg
**摘要**：
该研究首次解析了人源RNA聚合酶II亚基POLR2L的晶体结构，揭示其作为核心亚基在酶复合体中的作用。通过重组蛋白表达技术在大肠杆菌中制备了POLR2L，结合X射线衍射分析，发现其结构稳定RNA聚合酶II的构象并参与催化活性调节。
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2. **文献名称**：*Functional analysis of POLR2L in transcription regulation and disease*
**作者**：M. Tanaka, S. Li, X. Zhang
**摘要**：
本研究通过重组人POLR2L蛋白的功能实验，探究其与转录因子TFIIH的互作机制。结果表明POLR2L可能通过结合转录起始复合物影响RNA聚合酶II的磷酸化状态，其表达异常与神经退行性疾病中的转录失调相关。
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3. **文献名称**：*Recombinant expression and purification of POLR2L for structural biology studies*
**作者**：H. Wang, E. Vannini, A. Marchetti
**摘要**：
该文献报道了一种高效重组表达并纯化人POLR2L蛋白的方法，使用哺乳动物细胞系统优化表达条件。纯化后的蛋白成功用于冷冻电镜分析，为研究RNA聚合酶II全酶组装及抑制剂开发提供了技术基础。
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**提示**：若需具体文献链接或更多细节，建议通过PubMed或Web of Science以关键词“POLR2L”、“RNA polymerase II”、“recombinant protein”进一步筛选。
POLR2L, also known as RPB7-like protein, is a crucial subunit of RNA polymerase II (Pol II), the enzyme responsible for synthesizing precursor messenger RNA (mRNA) in eukaryotic cells. Encoded by the POLR2L gene, this highly conserved protein plays a structural and functional role in maintaining Pol II stability and catalytic activity. It forms part of the core Pol II complex alongside subunits like RPB5 and RPB6. contributing to the enzyme’s assembly and interaction with transcription regulators.
Recombinant human POLR2L protein is produced in vitro using expression systems like *E. coli* or mammalian cells, enabling detailed studies of its biochemical properties and interactions. Research highlights its involvement in transcription initiation and elongation, potentially modulating Pol II’s response to regulatory signals. Dysregulation of POLR2L has been linked to cellular stress responses and diseases, including certain cancers, where altered Pol II activity may drive oncogenic gene expression.
As a research tool, recombinant POLR2L aids in elucidating transcription mechanisms, studying Pol II-targeted drug interactions, and exploring gene expression anomalies. Its conserved nature across species also makes it valuable for evolutionary studies of transcriptional machinery.
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