| 纯度 | > 85 % SDS-PAGE |
| 种属 | Human |
| 靶点 | CCNB |
| Uniprot No | P14635 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-433aa |
| 氨基酸序列 | MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV |
| 预测分子量 | 50.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNB(Cyclin B)重组蛋白的3-4篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant Human Cyclin B1 in Escherichia coli*
**作者**：Li, X., Wang, Y., & Zhang, H.
**摘要**：该研究描述了在大肠杆菌中高效表达和纯化人源CCNB1重组蛋白的优化方法，通过亲和层析技术获得高纯度蛋白，并验证其在细胞周期调控相关实验中的活性。
2. **文献名称**：*Role of Recombinant Cyclin B-CDK1 Complex in Mitotic Entry*
**作者**：Morgan, D.O., & Pines, J.
**摘要**：文章利用重组CCNB1与CDK1蛋白复合体，阐明了其在调控有丝分裂进入中的分子机制，发现磷酸化修饰对复合体活性具有关键作用。
3. **文献名称**：*Recombinant Cyclin B as a Biomarker in Cancer Therapeutics*
**作者**：Chen, L., et al.
**摘要**：研究探讨了重组CCNB蛋白作为肿瘤治疗靶点的潜力，通过体外实验证明抑制CCNB可阻断癌细胞增殖，为药物筛选提供了模型。
4. **文献名称**：*Structural Analysis of Recombinant Cyclin B Using Cryo-EM*
**作者**：Yamaguchi, S., & Nogales, E.
**摘要**：通过冷冻电镜技术解析了重组CCNB蛋白的三维结构，揭示了其与CDK1结合的关键结构域，为靶向药物设计提供依据。
(注：以上文献为示例，实际引用时需根据真实研究补充信息。)
**Background of CCNB Recombinant Protein**
Cyclin B (CCNB) is a key regulatory protein involved in cell cycle control, specifically governing the transition from the G2 phase to mitosis (M phase) through its interaction with cyclin-dependent kinase 1 (CDK1). This complex, known as the Maturation-Promoting Factor (MPF), triggers critical events such as nuclear envelope breakdown, chromatin condensation, and spindle assembly. Dysregulation of CCNB expression or activity is linked to uncontrolled cell proliferation, genomic instability, and cancer progression, making it a focal point in oncology and cell biology research.
Recombinant CCNB proteins are engineered using genetic cloning techniques, where the *CCNB* gene is inserted into expression vectors (e.g., bacterial, insect, or mammalian systems) to produce high-purity, functional proteins. These recombinant variants retain the structural and functional properties of native Cyclin B, enabling researchers to study its role in cell cycle mechanisms, screen for CDK1 inhibitors, or explore therapeutic strategies targeting mitosis.
Applications of CCNB recombinant proteins span *in vitro* kinase assays, cell cycle synchronization studies, and drug discovery platforms. They are also utilized in developmental biology to investigate embryogenesis and tissue regeneration. Additionally, recombinant CCNB serves as an antigen for antibody production or diagnostic tools in cancer biomarker studies.
The use of recombinant technology ensures batch-to-batch consistency, eliminates contamination risks from native sources, and allows species-specific customization (e.g., human, mouse, or rat CCNB). Advances in protein engineering further enable post-translational modifications (e.g., phosphorylation) to mimic physiological conditions. Overall, CCNB recombinant proteins are indispensable tools for unraveling cell cycle dynamics and developing targeted cancer therapies.
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