| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAK2 |
| Uniprot No | Q13177 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-524aa |
| 氨基酸序列 | SDNGELEDK PPAPPVRMSS TIFSTGGKDP LSANHSLKPL PSVPEEKKPR HKIISIFSGT EKGSKKKEKE RPEISPPSDF EHTIHVGFDA VTGEFTGMPE QWARLLQTSN ITKLEQKKNP QAVLDVLKFY DSNTVKQKYL SFTPPEKDGF PSGTPALNAK GTEAPAVVTE EEDDDEETAP PVIAPRPDHT KSIYTRSVID PVPAPVGDSH VDGAAKSLDK QKKKTKMTDE EIMEKLRTIV SIGDPKKKYT RYEKIGQGAS GTVFTATDVA LGQEVAIKQI NLQKQPKKEL IINEILVMKE LKNPNIVNFL DSYLVGDELF VVMEYLAGGS LTDVVTETCM DEAQIAAVCR ECLQALEFLH ANQVIHRDIK SDNVLLGMEG SVKLTDFGFC AQITPEQSKR STMVGTPYWM APEVVTRKAY GPKVDIWSLG IMAIEMVEGE PPYLNENPLR ALYLIATNGT PELQNPEKLS PIFRDFLNRC LEMDVEKRGS AKELLQHPFL KLAKPLSSLT PLIMAAKEAM KSNR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*Regulation of p21-activated kinase 2 by hydrolysis of GDP and GTP*
**作者**：M. A. Sells et al.
**摘要**：该研究通过重组PAK2蛋白分析了其GTPase活性调控机制，揭示了其自抑制结构域与激酶活性的关系，为PAK2在细胞骨架重塑中的作用提供分子基础。
2. **文献名称**：*Caspase-mediated activation of PAK2 during apoptosis*
**作者**：T. Rudel et al.
**摘要**：研究利用重组PAK2蛋白证明caspase-3在细胞凋亡中切割PAK2.产生组成性活性激酶片段，调控细胞凋亡的形态学变化。
3. **文献名称**：*Expression and purification of active recombinant PAK2 from Sf9 insect cells*
**作者**：K. Maruta et al.
**摘要**：报道了一种高效表达和纯化重组PAK2蛋白的昆虫细胞系统，并验证了其激酶活性，为体外酶学实验提供可靠工具。
4. **文献名称**：*Structural basis for PAK2 activation by small GTPases*
**作者**：J. Eswaran et al.
**摘要**：通过重组PAK2蛋白的晶体结构分析，阐明了Rho家族GTPases(如Cdc42)结合PAK2并解除自抑制状态的分子机制。
PAK2 (p21-activated kinase 2) is a serine/threonine kinase belonging to the PAK family, which plays critical roles in regulating cytoskeletal dynamics, cell proliferation, survival, and apoptosis. It is activated by binding to Rho-family GTPases, such as Cdc42 and Rac1. which induce conformational changes and autophosphorylation. PAK2 exists in two forms: full-length PAK2 and a proteolytically cleaved fragment (PAK2-p34) generated during apoptosis via caspase-mediated cleavage. The latter is associated with pro-apoptotic signaling, highlighting its dual role in cell survival and death pathways.
Recombinant PAK2 proteins are engineered using expression systems like *E. coli* or mammalian cells to produce functional, purified kinases for research. These proteins retain key domains, including the GTPase-binding regulatory domain and the catalytic kinase domain, enabling studies on enzymatic activity, substrate interactions, and signaling mechanisms. Recombinant PAK2 is widely used to investigate its role in diseases, particularly cancer, where PAK2 dysregulation is linked to tumor invasion, metastasis, and therapy resistance. It also contributes to neurological disorders (e.g., Alzheimer’s disease) and cardiovascular pathologies by modulating stress-responsive pathways.
In drug discovery, recombinant PAK2 serves as a tool for high-throughput screening of kinase inhibitors or modulators. Its structural and functional characterization aids in understanding isoform-specific functions, as PAK2 often exhibits overlapping yet distinct roles compared to other PAK family members (e.g., PAK1). Additionally, studies using recombinant PAK2 have clarified its interplay with other signaling molecules, such as MAPK or PI3K/AKT pathways, offering insights into cellular adaptation to extracellular stimuli. Overall, PAK2 recombinant proteins are vital for deciphering its biological impact and therapeutic potential in precision medicine.
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