| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PES1 |
| Uniprot No | O00541 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588aa |
| 氨基酸序列 | MGGLEKKKYERGSATNYITRNKARKKLQLSLADFRRLCILKGIYPHEPKHKKKVNKGSTAARTFYLIKDIRFLLHEPIVNKFREYKVFVRKLRKAYGKSEWNTVERLKDNKPNYKLDHIIKERYPTFIDALRDLDDALSMCFLFSTFPRTGKCHVQTIQLCRRLTVEFMHYIIAARALRKVFLSIKGIYYQAEVLGQPIVWITPYAFSHDHPTDVDYRVMATFTEFYTTLLGFVNFRLYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALSASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSFGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLPPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEKEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIREANKLAEKRKAHDEAVRSEKKAKKARPE |
| 预测分子量 | 75.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设存在的关于PES1重组蛋白的3篇参考文献示例(注：文献信息为虚构，仅供格式参考)：
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1. **文献名称**: *"Recombinant PES1 Protein Expression and Its Role in Ribosome Biogenesis"*
**作者**: Smith A, et al.
**期刊/年份**: *Journal of Biological Chemistry*, 2018
**摘要**: 研究利用大肠杆菌系统成功表达并纯化重组人源PES1蛋白，证明其通过调控核糖体RNA加工参与细胞增殖，缺失实验表明PES1与癌症相关通路存在关联。
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2. **文献名称**: *"Structural Analysis of PES1 and Its Interaction with BOP1 in the PeBoW Complex"*
**作者**: Zhang L, et al.
**期刊/年份**: *Cell Reports*, 2020
**摘要**: 通过重组PES1蛋白的晶体结构解析，揭示了其与BOP1和WDR12形成PeBoW复合物的关键结构域，为靶向该复合物抑制肿瘤生长提供了理论依据。
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3. **文献名称**: *"PES1 Recombinant Protein Promotes Breast Cancer Metastasis via AKT Signaling"*
**作者**: Kim Y, et al.
**期刊/年份**: *Oncogene*, 2021
**摘要**: 体外实验显示重组PES1蛋白通过激活AKT/mTOR通路增强乳腺癌细胞侵袭能力，体内模型进一步证实其促进肿瘤转移的功能，提示PES1为潜在治疗靶点。
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**注**：实际文献需通过PubMed、Web of Science等数据库检索关键词(如PES1 recombinant protein, Pescadillo cancer)，推荐结合具体研究方向筛选。
PES1 (Pescadillo 1) is a multifunctional nucleolar protein implicated in ribosome biogenesis, cell cycle regulation, and cancer progression. It was initially identified in zebrafish for its role in embryonic development, and homologs exist across eukaryotes. Structurally, PES1 contains a conserved N-terminal domain and a BRCA1 C-terminal (BRCT) domain, facilitating interactions with proteins like Bop1 and WDR12 to form the PeBoW complex, essential for rRNA processing and ribosome assembly.
Recombinant PES1 protein is produced using expression systems like *E. coli* or mammalian cells, often tagged with His or GST for purification. Its recombinant form enables studies on molecular mechanisms, including nucleolar stress responses, DNA repair, and cell proliferation. PES1 overexpression is linked to tumorigenesis in cancers such as breast, liver, and colorectal, where it promotes oncogenic pathways (e.g., Wnt/β-catenin) and inhibits apoptosis. This makes it a potential therapeutic target or diagnostic biomarker.
Research on recombinant PES1 also explores its role in stem cell maintenance and aging. For example, it stabilizes telomerase reverse transcriptase (TERT), influencing telomere dynamics. However, its dual functions in ribosome synthesis and extranucleolar processes pose challenges in dissecting context-dependent roles. Current studies employ knockouts, RNAi, or recombinant protein supplementation to clarify its regulatory networks.
Despite progress, questions remain about post-translational modifications, tissue-specific interactions, and therapeutic targeting strategies. Recombinant PES1 tools continue to advance structural analyses and high-throughput screening for inhibitors, bridging basic biology and translational applications.
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