| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PODN |
| Uniprot No | Q7Z5L7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-613aa |
| 氨基酸序列 | G PVLAVRAPGF GRSGGHSLSP EENEFAEEEP VLVLSPEEPG PGPAAVSCPR DCACSQEGVV DCGGIDLREF PGDLPEHTNH LSLQNNQLEK IYPEELSRLH RLETLNLQNN RLTSRGLPEK AFEHLTNLNY LYLANNKLTL APRFLPNALI SVDFAANYLT KIYGLTFGQK PNLRSVYLHN NKLADAGLPD NMFNGSSNVE VLILSSNFLR HVPKHLPPAL YKLHLKNNKL EKIPPGAFSE LSSLRELYLQ NNYLTDEGLD NETFWKLSSL EYLDLSSNNL SRVPAGLPRS LVLLHLEKNA IRSVDANVLT PIRSLEYLLL HSNQLREQGI HPLAFQGLKR LHTVHLYNNA LERVPSGLPR RVRTLMILHN QITGIGREDF ATTYFLEELN LSYNRITSPQ VHRDAFRKLR LLRSLDLSGN RLHTLPPGLP RNVHVLKVKR NELAALARGA LVGMAQLREL YLTSNRLRSR ALGPRAWVDL AHLQLLDIAG NQLTEIPEGL PESLEYLYLQ NNKISAVPAN AFDSTPNLKG IFLRFNKLAV GSVVDSAFRR LKHLQVLDIE GNLEFGDISK DRGRLGKEKE EEEEEEEEEE ETR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PODN(Podocan)重组蛋白研究的示例参考文献(注：以下为模拟示例，实际文献需通过学术数据库检索)：
1. **文献名称**: "Podocan重组蛋白抑制肝癌细胞迁移的机制研究"
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌表达系统制备重组PODN蛋白，发现其能通过调控Wnt/β-catenin通路显著抑制肝癌细胞的迁移和侵袭能力，为肝癌治疗提供潜在靶点。
2. **文献名称**: "重组Podocan蛋白在肺纤维化中的抗纤维化作用"
**作者**: Wang Y, et al.
**摘要**: 研究利用哺乳动物细胞表达体系获得高纯度PODN重组蛋白，证实其可通过抑制TGF-β1/Smad3信号通路减轻小鼠肺纤维化模型中的胶原沉积和炎症反应。
3. **文献名称**: "Podocan重组表达及其在乳腺癌血管生成中的功能分析"
**作者**: Chen H, et al.
**摘要**: 通过昆虫细胞表达系统制备功能性PODN重组蛋白，发现其能抑制血管内皮细胞管腔形成，并降低VEGF表达水平，提示其在抑制乳腺癌血管生成中的作用。
4. **文献名称**: "重组Podocan蛋白对软骨细胞分化的调控研究"
**作者**: Kim S, et al.
**摘要**: 研究利用真核表达系统获得PODN重组蛋白，证明其通过调控SOX9和COL2A1表达抑制软骨细胞过度分化，为骨关节炎治疗提供新思路。
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**建议**：实际研究中可通过PubMed、Web of Science等平台检索关键词"Podocan recombinant protein"或"PODN expression and function"，重点关注近5年发表的文献以获取最新进展。
**Background of PODN Recombinant Protein**
PODN (Podocan) is a small leucine-rich repeat proteoglycan (SLRP) encoded by the *PODN* gene, primarily expressed in connective tissues and vascular smooth muscle cells. It plays a regulatory role in extracellular matrix (ECM) organization, cell adhesion, and signaling pathways. Structurally, PODN contains leucine-rich repeats (LRRs) critical for protein-protein interactions and a conserved C-terminal domain that may modulate collagen fibrillogenesis. Studies suggest PODN influences cellular processes like proliferation, migration, and differentiation, with implications in fibrosis, atherosclerosis, and tumorigenesis.
Recombinant PODN protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, bioactive forms for research. This allows precise study of its interactions with ECM components (e.g., collagens, growth factors) and receptors (e.g., integrins). Applications include investigating its role in diseases: upregulated PODN in fibrotic tissues correlates with ECM remodeling, while its anti-angiogenic properties in cancer models highlight therapeutic potential. Additionally, recombinant PODN serves as a tool to explore mechanotransduction and tissue repair mechanisms.
Despite progress, functional nuances of PODN remain understudied. Its dual roles in promoting or inhibiting pathological processes, depending on context, underscore the need for further research. Recombinant protein technology enables scalable production for *in vitro* and *in vivo* studies, aiding drug discovery and biomarker development. Overall, PODN recombinant protein is a valuable resource for deciphering ECM biology and its disease-related dysregulation.
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