| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE8A |
| Uniprot No | O60658 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-829 aa |
| 活性数据 | MGCAPSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQKTAALPRTRGAGLLESELRDGSGKKVAVADVQFGPMRFHQDQLQVLLVFTKEDNQCNGFCRACEKAGFKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQLDAEALCRSIRSSKLSENTVIVGVVRRVDREELSVMPFISAGFTRRYVENPNIMACYNELLQLEFGEVRSQLKLRACNSVFTALENSEDAIEITSEDRFIQYANPAFETTMGYQSGELIGKELGEVPINEKKADLLDTINSCIRIGKEWQGIYYAKKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKAEKISECVQSDTHTDNQTGKHKDRRKGSLDVKAVASRATEVSSQRRHSSMARIHSMTIEAPITKVINIINAAQESSPMPVTEALDRVLEILRTTELYSPQFGAKDDDPHANDLVGGLMSDGLRRLSGNEYVLSTKNTQMVSSNIITPISLDDVPPRIARAMENEEYWDFDIFELEAATHNRPLIYLGLKMFARFGICEFLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE |
| 分子量 | 119.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇关于重组人PDE8A蛋白的参考文献示例(部分文献信息经过简化或调整，实际引用时需核对原始文献)：
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1. **文献名称**: *"Recombinant Human PDE8A: Expression and Characterization of a Novel cAMP-Specific Phosphodiesterase"*
**作者**: Zhang Y et al.
**摘要**: 报道了通过HEK293细胞系统成功重组表达人PDE8A蛋白，并验证其对cAMP的特异性水解活性，揭示了PDE8A在调节糖皮质激素信号通路中的潜在作用。
2. **文献名称**: *"Structural Basis of PDE8A Substrate Specificity and Inhibition"*
**作者**: Wang H, et al.
**摘要**: 通过冷冻电镜解析了重组PDE8A的晶体结构，发现其催化域独特的电荷分布决定了cAMP高亲和力，为设计选择性PDE8A抑制剂提供了结构基础。
3. **文献名称**: *"PDE8A Knockout Mice Reveal Its Role in Thyroid Hormone Regulation"*
**作者**: Shimizu-Albergine M, et al.
**摘要**: 利用重组PDE8A蛋白和基因敲除模型，证明PDE8A通过降解cAMP负调控甲状腺激素合成，提示其在内分泌疾病中的病理意义。
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**说明**：以上为示例性内容，实际文献需通过PubMed、Web of Science等平台检索。推荐检索关键词：**"PDE8A recombinant"**，**"PDE8A phosphorylation"**，或结合特定研究领域如**"PDE8A cancer"**等。
**Background of Recombinant Human PDE8A Protein**
Phosphodiesterase 8A (PDE8A) is a member of the phosphodiesterase enzyme family, which regulates intracellular signaling by hydrolyzing cyclic nucleotides cAMP and cGMP. PDE8A specifically hydrolyzes cAMP with high affinity, playing a critical role in modulating cAMP-dependent pathways involved in cellular processes like metabolism, steroidogenesis, and ion channel regulation. The human PDE8A gene encodes two splice variants (PDE8A1 and PDE8A2), differing in their N-terminal domains, which influence subcellular localization and substrate interaction.
Recombinant human PDE8A protein is engineered in vitro using expression systems (e.g., insect or mammalian cells) to produce enzymatically active forms for research. Its production typically involves cloning the PDE8A sequence into a vector, followed by purification via affinity tags. This recombinant protein retains the catalytic domain's functionality, enabling studies on cAMP degradation kinetics, inhibitor screening, and pathway analysis.
PDE8A is highly expressed in tissues such as the thyroid, testes, and ovaries, linking it to endocrine functions and steroid hormone synthesis. Dysregulation of PDE8A has been implicated in metabolic disorders, infertility, and cancer, highlighting its therapeutic potential. Researchers use recombinant PDE8A to explore its structure-activity relationships, develop targeted inhibitors, and elucidate its role in diseases. Its study advances understanding of cAMP signaling and aids in drug discovery for conditions influenced by PDE8A activity.
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