| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PYGO1 |
| Uniprot No | Q9Y3Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MPAENSPAPA YKVSSHGGDS GLDGLGGPGV QLGSPDKKKR KANTQGPSFP PLSEYAPPPN PNSDHLVAAN PFDDNYNTIS YKPLPSSNPY LGPGYPGFGG YSTFRMPPHV PPRMSSPYCG PYSLRNQPHP FPQNPLGMGF NRPHAFNFGP HDNSSFGNPS YNNALSQNVN MPNQHFRQNP AENFSQIPPQ NASQVSNPDL ASNFVPGNNS NFTSPLESNH SFIPPPNTFG QAKAPPPKQD FTQGATKNTN QNSSAHPPHL NMDDTVNQSN IELKNVNRNN AVNQENSRSS STEATNNNPA NGTQNKPRQP RGAADACTTE KSNKSSLHPN RHGHSSSDPV YPCGICTNEV NDDQDAILCE ASCQKWFHRI CTGMTETAYG LLTAEASAVW GCDTCMADKD VQLMRTRETF GPSAVGSDA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYGO1重组蛋白的3篇模拟参考文献，基于领域内常见研究方向整理而成：
---
1. **文献名称**：*PYGO1 promotes colorectal cancer progression through Wnt/β-catenin signaling via interaction with histone H3K4me2*
**作者**：Zhang L, Chen X, Wang Y
**摘要**：本研究通过重组PYGO1蛋白的体外实验，发现其通过结合组蛋白H3K4me2修饰位点，增强β-catenin的转录活性，激活Wnt信号通路，从而促进结直肠癌细胞增殖和迁移。
2. **文献名称**：*Structural characterization of recombinant PYGO1 and its role in chromatin remodeling*
**作者**：Smith J, Brown K, Lee M
**摘要**：利用大肠杆菌表达系统纯化重组PYGO1蛋白，结合X射线晶体学解析其三维结构，揭示了其HD1结构域与组蛋白结合的关键位点，为靶向抑制PYGO1的癌症治疗策略提供依据。
3. **文献名称**：*PYGO1 overexpression enhances breast cancer stem cell properties via epigenetic regulation*
**作者**：Wang H, Li T, Garcia-Reyes B
**摘要**：通过重组PYGO1蛋白的细胞导入实验，证明其通过招募组蛋白甲基转移酶复合物，维持乳腺癌干细胞表观遗传状态，促进肿瘤干性特征和化疗耐药性。
---
**备注**：以上文献为模拟示例，实际引用时建议通过PubMed、Google Scholar等平台检索真实论文，关键词可包括“PYGO1 recombinant protein”“PYGO1 Wnt signaling”等。
PYGO1 (Pygopus family PHD finger 1) is a chromatin-associated protein that functions as a coactivator in the Wnt/β-catenin signaling pathway, a critical pathway regulating embryonic development, stem cell maintenance, and cancer progression. It belongs to the Pygopus family, characterized by a conserved plant homeodomain (PHD) finger involved in recognizing histone modifications, particularly trimethylated histone H3 lysine 4 (H3K4me3), and a C-terminal homology domain for protein interactions. PYGO1 stabilizes the β-catenin/TCF transcription complex at Wnt target gene promoters, enhancing transcriptional activation.
Recombinant PYGO1 proteins are engineered in vitro using expression systems like *E. coli* or mammalian cell lines, often fused with tags (e.g., His, GST) for purification and detection. These proteins are indispensable tools for studying PYGO1’s structure-function relationships, its role in Wnt signaling dysregulation in cancers (e.g., colorectal, breast), and its interplay with chromatin modifiers like BCL9 or histone demethylases. Studies using recombinant PYGO1 have revealed its oncogenic potential, including promoting cell proliferation, metastasis, and chemoresistance. Researchers also employ it for drug screening to identify inhibitors targeting PYGO1’s PHD domain or β-catenin-binding interface. Despite progress, questions remain about its tissue-specific roles and post-translational regulation, driving ongoing interest in recombinant PYGO1 for mechanistic and therapeutic exploration.
×
