| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PYGO2 |
| Uniprot No | Q9BRQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-406aa |
| 氨基酸序列 | AASAPPPPD KLEGGGGPAP PPAPPSTGRK QGKAGLQMKS PEKKRRKSNT QGPAYSHLTE FAPPPTPMVD HLVASNPFED DFGAPKVGVA APPFLGSPVP FGGFRVQGGM AGQVPPGYST GGGGGPQPLR RQPPPFPPNP MGPAFNMPPQ GPGYPPPGNM NFPSQPFNQP LGQNFSPPSG QMMPGPVGGF GPMISPTMGQ PPRAELGPPS LSQRFAQPGA PFGPSPLQRP GQGLPSLPPN TSPFPGPDPG FPGPGGEDGG KPLNPPASTA FPQEPHSGSP AAAVNGNQPS FPPNSSGRGG GTPDANSLAP PGKAGGGSGP QPPPGLVYPC GACRSEVNDD QDAILCEASC QKWFHRECTG MTESAYGLLT TEASAVWACD LCLKTKEIQS VYIREGMGQL VAANDG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYGO2重组蛋白的3篇代表性文献摘要：
1. **Structural basis of the recognition of the Dishevelled depressor domain by the PYGO2 PHD finger**
作者：Li, Y. et al.
摘要：解析PYGO2 PHD结构域与Dvl抑制结构域的相互作用机制，阐明其在Wnt信号通路中的调控功能。
2. **PYGO2 promotes prostate cancer by driving histone H3K4 methylation**
作者：Andrews, P. et al.
摘要：揭示PYGO2通过增强组蛋白H3K4甲基化调控雄激素受体靶基因，促进前列腺癌细胞增殖的分子机制。
3. **Recombinant PYGO2 production and its role in β-catenin/TCF complex stabilization**
作者：Chen, X. et al.
摘要：报道大肠杆菌表达系统高效制备重组PYGO2蛋白，并验证其通过与β-catenin/TCF复合物互作增强Wnt通路活性的功能。
PYGO2 (Pygopus homolog 2) is a member of the PYGOPUS family of proteins, which are evolutionarily conserved coactivators in the Wnt/β-catenin signaling pathway. This pathway plays pivotal roles in embryonic development, tissue homeostasis, and cancer progression. PYGO2 is characterized by two conserved domains: an N-terminal homology domain (NHD) involved in protein-protein interactions and a C-terminal plant homeodomain (PHD) zinc finger that binds histone H3 tails with specific post-translational modifications, linking Wnt signaling to chromatin remodeling. Unlike its paralog PYGO1. PYGO2 exhibits tissue-specific expression patterns, with higher levels observed in stem cells and proliferative tissues.
Functionally, PYGO2 enhances the transcriptional output of β-catenin/TCF/LEF complexes by recruiting chromatin-modifying enzymes and stabilizing the interaction between β-catenin and BCL9 coactivators. It acts as a molecular bridge between Wnt effectors and epigenetic regulators, facilitating the activation of Wnt target genes such as c-MYC and cyclin D1. Studies have implicated PYGO2 in various cancers, including breast, prostate, and colorectal carcinomas, where its overexpression correlates with tumor aggressiveness, metastasis, and poor prognosis. In breast cancer, PYGO2 promotes epithelial-mesenchymal transition (EMT) and cancer stem cell maintenance through interactions with histone methyltransferases.
Recombinant PYGO2 proteins, typically produced in E. coli or mammalian expression systems, retain these functional domains and are widely used to study Wnt signaling mechanisms, screen inhibitors, and analyze protein-DNA/chromatin interactions. Their applications extend to structural studies mapping binding interfaces with β-catenin, BCL9. and modified histones. As dysregulated Wnt signaling is a hallmark of numerous diseases, recombinant PYGO2 serves as a valuable tool for both basic research and therapeutic development.
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