| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PATZ1 |
| Uniprot No | Q9HBE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-687 aa |
| 活性数据 | MERVNDASCG PSGCYTYQVS RHSTEMLHNL NQQRKNGGRF CDVLLRVGDE SFPAHRAVLA ACSEYFESVF SAQLGDGGAA DGGPADVGGA TAAPGGGAGG SRELEMHTIS SKVFGDILDF AYTSRIVVRL ESFPELMTAA KFLLMRSVIE ICQEVIKQSN VQILVPPARA DIMLFRPPGT SDLGFPLDMT NGAALAANSN GIAGSMQPEE EAARAAGAAI AGQASLPVLP GVDRLPMVAG PLSPQLLTSP FPSVASSAPP LTGKRGRGRP RKANLLDSMF GSPGGLREAG ILPCGLCGKV FTDANRLRQH EAQHGVTSLQ LGYIDLPPPR LGENGLPISE DPDGPRKRSR TRKQVACEIC GKIFRDVYHL NRHKLSHSGE KPYSCPVCGL RFKRKDRMSY HVRSHDGSVG KPYICQSCGK GFSRPDHLNG HIKQVHTSER PHKCQTCNAS FATRDRLRSH LACHEDKVPC QVCGKYLRAA YMADHLKKHS EGPSNFCSIC NRGFSSASYL KVHVKTHHGV PLPQVSRHQE PILNGGAAFH CARTYGNKEG QKCSHQDPIE SSDSYGDLSD ASDLKTPEKQ SANGSFSCDM AVPKNKMESD GEKKYPCPEC GSFFRSKSYL NKHIQKVHVR ALGGPLGDLG PALGSPFSPQ QNMSLLESFG FQIVQSAFAS SLVDPEVDQQ PMGPEGK |
| 分子量 | 74.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PATZ1蛋白功能及机制的关键文献概览：
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1. **文献名称**：*PATZ1 functions as a tumor suppressor in glioblastoma by transcriptional repression of multiple oncogenic genes*
**作者**：Mastropietro G, et al.
**摘要**：该研究利用重组PATZ1蛋白揭示了其在胶质母细胞瘤中的抑癌功能，通过结合特定启动子区域抑制致癌基因(如MYC)的转录活性，并证实其缺失导致肿瘤细胞增殖与侵袭性增强。
2. **文献名称**：*The POZ/BTB domain of PATZ1 mediates transcriptional repression and interaction with the NuRD complex*
**作者**：Fedele M, et al.
**摘要**：研究通过重组PATZ1的POZ/BTB结构域，解析其作为转录抑制因子的分子机制，包括通过招募NuRD复合体实现染色质重塑，从而调控胚胎干细胞多能性相关基因的表达沉默。
3. **文献名称**：*PATZ1 interacts with p53 and modulates its transcriptional activity in cancer cell lines*
**作者**：Valentino T, et al.
**摘要**：此文献证明重组PATZ1蛋白在结肠癌细胞中与p53直接结合，增强p53靶基因的转录激活能力，并抑制肿瘤生长，提示PATZ1-p53通路在癌症治疗中的潜在应用价值。
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**备注**：若需获取全文，可结合上述标题及作者信息在PubMed或Sci-Hub平台检索。研究方向建议关注PATZ1在转录调控、癌症及干细胞中的动态互作网络。
**Background of Recombinant Human PATZ1 Protein**
PATZ1 (POZ/BTB and AT-Hook-containing Zinc Finger 1), also known as ZBTB49. is a transcription factor encoded by the *PATZ1* gene. It belongs to the zinc finger protein family, characterized by a unique structure containing a POZ/BTB domain for protein-protein interactions and multiple zinc finger motifs for DNA binding. PATZ1 regulates gene expression by acting as a transcriptional repressor or activator, depending on cellular context and binding partners.
Biologically, PATZ1 plays critical roles in cell differentiation, proliferation, and apoptosis. It is implicated in embryonic development, particularly in neural and reproductive systems, and maintains stem cell pluripotency. Dysregulation of PATZ1 is linked to cancer, where it may act as a tumor suppressor or oncogene. For instance, it interacts with p53 to modulate apoptosis and participates in the NF-κB pathway to influence immune responses.
Recombinant human PATZ1 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study its molecular functions, DNA-binding properties, or therapeutic potential. It serves as a tool for investigating PATZ1’s role in diseases and developing targeted therapies. Research on PATZ1 continues to uncover its complex regulatory networks and clinical relevance in oncology and regenerative medicine.
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