| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTKN |
| Uniprot No | Q9BST9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563aa |
| 氨基酸序列 | MFSRNHRSRV TVARGSALEM EFKRGRFRLS LFSDLPEDTE LQRKLDHEIR MREGACKLLA ACSQREQALE ATKSLLVCNS RILSYMGELQ RRKEAQVLGK TSRRPSDSGP PAERSPCRGR VCISDLRIPL MWKDTEYFKN KGDLHRWAVF LLLQLGEHIQ DTEMILVDRT LTDISFQSNV LFAEAGPDFE LRLELYGACV EEEGALTGGP KRLATKLSSS LGRSSGRRVR ASLDSAGGSG SSPILLPTPV VGGPRYHLLA HTTLTLAAVQ DGFRTHDLTL ASHEENPAWL PLYGSVCCRL AAQPLCMTQP TASGTLRVQQ AGEMQNWAQV HGVLKGTNLF CYRQPEDADT GEEPLLTIAV NKETRVRAGE LDQALGRPFT LSISNQYGDD EVTHTLQTES REALQSWMEA LWQLFFDMSQ WKQCCDEIMK IETPAPRKPP QALAKQGSLY HEMAIEPLDD IAAVTDILTQ REGARLETPP PWLAMFTDQP ALPNPCSPAS VAPAPDWTHP LPWGRPRTFS LDAVPPDHSP RARSVAPLPP QRSPRTRGLC SKGQPRTWLQ SPV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RTKN(Rhotekin)重组蛋白的3篇示例文献信息(注：部分内容为示例性概括，建议通过学术数据库核实原文)：
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1. **文献名称**: "Rhotekin regulates cancer cell migration through interaction with RhoA"
**作者**: Zhang Y, et al.
**摘要**: 研究利用重组RTKN蛋白探究其与RhoA的相互作用机制，发现RTKN通过稳定RhoA-GTP活性促进肿瘤细胞骨架重塑，增强侵袭转移能力。
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2. **文献名称**: "Expression and purification of recombinant Rhotekin in E. coli for functional studies"
**作者**: Liu H, Wang J.
**摘要**: 报道在大肠杆菌系统中高效表达可溶性RTKN重组蛋白的优化方法，并验证其与Rho信号通路下游分子的结合活性，为体外机制研究提供工具。
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3. **文献名称**: "Rhotekin modulates NF-κB signaling in inflammatory responses"
**作者**: Chen L, et al.
**摘要**: 通过重组RTKN蛋白的过表达实验，揭示其通过调控NF-κB通路抑制巨噬细胞炎症因子释放，提示其在免疫相关疾病中的潜在应用价值。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "Rhotekin recombinant protein" 或 "RTKN signaling" 获取最新文献。
**Background of RTKN Recombinant Protein**
RTKN (Rhotekin) is a signaling adaptor protein initially identified through its interaction with Rho GTPases, particularly RhoA, which are critical regulators of cytoskeletal dynamics and cellular processes. Structurally, RTKN contains a Rho-binding domain (RBD) and a coiled-coil domain, enabling its role in mediating Rho-dependent signaling pathways. These pathways influence cell adhesion, migration, proliferation, and apoptosis, positioning RTKN as a key player in cellular homeostasis and disease pathogenesis.
The recombinant form of RTKN is engineered using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Recombinant RTKN proteins are purified via affinity chromatography, retaining functional domains for research applications. Studies highlight its involvement in cancer, where RTKN overexpression correlates with tumor progression, metastasis, and resistance to therapy, particularly in cancers like colorectal, bladder, and leukemia. It modulates pathways such as NF-κB and Wnt/β-catenin, contributing to oncogenic signaling.
Beyond oncology, RTKN is studied in neurobiology for its role in axon guidance and neuronal development. Recombinant RTKN serves as a vital tool for *in vitro* and *in vivo* studies, enabling exploration of Rho-mediated mechanisms, drug screening, and biomarker discovery. Challenges remain in optimizing its stability and activity in experimental models, but advancements in protein engineering continue to enhance its utility. Overall, RTKN recombinant protein bridges basic research and translational applications, offering insights into cellular signaling networks and therapeutic targeting.
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