| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIOK1 |
| Uniprot No | Q9BRS2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MDYRRLLMSR VVPGQFDDAD SSDSENRDLK TVKEKDDILF EDLQDNVNEN GEGEIEDEEE EGYDDDDDDW DWDEGVGKLA KGYVWNGGSN PQANRQTSDS SSAKMSTPAD KVLRKFENKI NLDKLNVTDS VINKVTEKSR QKEADMYRIK DKADRATVEQ VLDPRTRMIL FKMLTRGIIT EINGCISTGK EANVYHASTA NGESRAIKIY KTSILVFKDR DKYVSGEFRF RHGYCKGNPR KMVKTWAEKE MRNLIRLNTA EIPCPEPIML RSHVLVMSFI GKDDMPAPLL KNVQLSESKA RELYLQVIQY MRRMYQDARL VHADLSEFNM LYHGGGVYII DVSQSVEHDH PHALEFLRKD CANVNDFFMR HSVAVMTVRE LFEFVTDPSI THENMDAYLS KAMEIASQRT KEERSSQDHV DEEVFKRAYI PRTLNEVKNY ERDMDIIMKL KEEDMAMNAQ QDNILYQTVT GLKKDLSGVQ KVPALLENQV EERTCSDSED IGSSECSDTD SEEQGDHARP KKHTTDPDID KKERKKMVKE AQREKRKNKI PKHVKKRKEK TAKTKKGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIOK1重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**：*RIOK1 kinase activity is required for mitotic progression and regulates chromosomal stability*
**作者**：E. A. Gallego-Paez et al.
**摘要**：研究利用重组RIOK1蛋白进行体外激酶活性分析，发现其磷酸化特定底物并参与调控有丝分裂进程。实验表明，RIOK1活性缺失导致染色体分离异常，提示其在维持基因组稳定性中的关键作用。
2. **文献名称**：*Structural and functional characterization of human RIOK1 reveals a conserved kinase scaffold*
**作者**：S. M. Heider et al.
**摘要**：通过重组表达并纯化人源RIOK1蛋白，结合X射线晶体学解析其三维结构，揭示其非典型激酶结构域特征。功能实验表明RIOK1通过保守的ATP结合位点调控核糖体生物合成通路。
3. **文献名称**：*Development of a high-throughput assay for RIOK1 inhibitors using recombinant protein*
**作者**：J. R. Thompson et al.
**摘要**：报道了基于重组RIOK1蛋白的激酶活性检测平台，用于筛选小分子抑制剂。研究发现多个候选化合物可特异性抑制RIOK1活性，为癌症靶向治疗提供潜在策略。
注：上述文献为示例性概括，实际文献需通过PubMed或Web of Science检索获取。建议结合关键词"RIOK1 recombinant protein"+"kinase activity"/"purification"/"crystal structure"进一步查阅。
RIOK1 (RIO kinase 1) is a member of the RIO kinase family, which includes atypical serine/threonine kinases involved in ribosome biogenesis, cell cycle regulation, and genome stability. Unlike conventional kinases, RIOK1 lacks canonical regulatory domains, yet it plays a critical role in pre-ribosomal RNA (rRNA) processing and the assembly of 40S ribosomal subunits. It is predominantly localized in the cytoplasm and nucleus, with its expression and activity tightly linked to cell proliferation. Studies suggest RIOK1 interacts with multiple components of the ribosome biogenesis machinery and may influence pathways beyond ribosome maturation, including mTOR signaling and cancer metastasis.
Recombinant RIOK1 protein is engineered for in vitro studies to dissect its kinase activity, structural features, and molecular interactions. Typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, the recombinant protein retains key functional domains, such as its N-terminal kinase domain and C-terminal region. Purification often involves affinity tags (e.g., His-tag) for streamlined isolation. Researchers utilize recombinant RIOK1 to investigate its enzymatic mechanisms, screen for kinase inhibitors, or explore its role in diseases like cancer, where RIOK1 overexpression correlates with poor prognosis in certain malignancies.
Despite progress, questions remain about RIOK1's substrate specificity, regulatory networks, and therapeutic potential. Its atypical kinase structure and dual roles in ribosome biogenesis and cell cycle control make it a unique target for studying cancer biology and developing targeted therapies. Current research focuses on elucidating its interplay with other RIO kinases (RIOK2/3) and validating its candidacy as a biomarker or drug target in oncology.
×
