| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR51B4 |
| Uniprot No | Q9Y5P0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310 aa |
| 活性数据 | MWYNNSAGPFLLTGFLGSEAVHYRISMSFFVIYFSVLFGNGTLLVLIWNDHSLHEPMYYF LAMLADTDLGMTFTTMPTVLGVLLLDQREIAHAACFTQSFIHSLAIVESGILLVLAYDCF IAIRTPLRYNCILTNSRVMNIGLGVLMRGFMSILPIILSLYCYPYCGSRALLHTFCLHQD VIKLACADITFNHIYPIIQTSLTVFLDALIIIFSYILILKTVMGIASGQEEAKSLNTCVS HISCVLVFHITVMGLSFIHRFGKHAPHVVPITMSYVHFLFPPFVNPIIYSIKTKQIQRSI IRLFSGQSRA |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR51B4蛋白的3-4条参考文献的简要列举：
1. **文献名称**：重组人OR51B4蛋白的异源表达及其配体结合特性分析
**作者**：Smith J, 等
**摘要**：该研究在昆虫细胞系统中成功表达并纯化了重组OR51B4蛋白，通过荧光钙流实验验证其与中链脂肪酸的特异性结合，揭示了其在非嗅觉组织中的潜在生理功能。
2. **文献名称**：OR51B4受体在胰腺癌中的功能及其分子机制
**作者**：Zhang L, 等
**摘要**：发现OR51B4在胰腺癌细胞中高表达，重组蛋白实验表明其通过激活MAPK通路促进肿瘤生长，为癌症治疗提供了新靶点。
3. **文献名称**：人嗅觉受体OR51B4的结构动态模拟与配体识别
**作者**：Wang Y, 等
**摘要**：利用分子动力学模拟解析OR51B4与癸酸复合物的构象变化，阐明其配体选择性机制，为基于结构的药物设计提供依据。
4. **文献名称**：OR51B4调控巨噬细胞炎症反应的代谢通路研究
**作者**：Chen R, 等
**摘要**：重组OR51B4蛋白实验证实其通过结合短链脂肪酸抑制巨噬细胞NLRP3炎症小体活化，提示其在免疫代谢疾病中的作用。
(注：上述文献为示例性内容，实际研究中需引用真实发表的论文。)
OR51B4 is a human olfactory receptor belonging to the G protein-coupled receptor (GPCR) superfamily, specifically classified under subfamily 51B. While primarily expressed in olfactory sensory neurons of the nasal epithelium, emerging studies suggest its ectopic expression in non-olfactory tissues, including prostate, brain, and gastrointestinal organs. As a class II olfactory receptor, it features seven transmembrane domains and is thought to detect specific odorant molecules, though its precise endogenous ligands remain largely uncharacterized (making it an orphan receptor).
Recombinant OR51B4 protein is produced through heterologous expression systems like HEK293 or bacterial cells, enabling structural and functional studies. Researchers employ it to investigate ligand-binding specificity, downstream signaling pathways (primarily involving cAMP modulation), and potential non-olfactory roles in cellular processes such as chemotaxis and apoptosis. Its ectopic expression has sparked interest in disease associations, with preliminary evidence linking OR51B4 dysregulation to prostate cancer progression and metabolic disorders.
Current challenges include elucidating activation mechanisms, identifying physiological agonists beyond odorants, and validating tissue-specific functions. Recombinant OR51B4 serves as a critical tool for developing biosensors, screening odorant libraries, and exploring therapeutic targeting of olfactory receptors in non-traditional contexts. Technical optimizations for protein stabilization and membrane reconstitution remain active areas of method development.
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