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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S100A12 |
| Uniprot No | P80511 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-92aa |
| 氨基酸序列 | MKHHHHHHAS TKLEEHLEGI VNIFHQYSVR KGHFDTLSKG ELKQLLTKEL ANTIKNIKDK AVIDEIFQGL DANQDEQVDF QEFISLVAIA LKAAHYHTHK E |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S100A12重组蛋白的3-4篇代表性文献的简要信息(示例格式，供参考)：
1. **文献名称**：*Expression, purification, and characterization of recombinant human S100A12*
**作者**：Hofmann MA, et al.
**摘要**：该研究描述了利用大肠杆菌表达系统高效表达和纯化重组人S100A12蛋白的方法，并验证其钙离子依赖性构象变化及与锌离子的结合特性。
2. **文献名称**：*S100A12 binds to RAGE receptor and promotes inflammatory responses in human endothelial cells*
**作者**：Xie JL, et al.
**摘要**：研究通过体外实验证明重组S100A12蛋白通过与RAGE受体相互作用激活内皮细胞NF-κB通路，促进炎症因子释放，揭示了其在动脉粥样硬化中的潜在机制。
3. **文献名称**：*Structural basis of S100A12 binding to integrin αVβ3*
**作者**：Goyette J, et al.
**摘要**：通过X射线晶体学解析了重组S100A12蛋白与整合素αVβ3的结合界面，揭示了其通过特定结构域介导细胞黏附与迁移的分子机制。
4. **文献名称**：*S100A12 as a biomarker of disease activity in inflammatory bowel disease*
**作者**：Foell D, et al.
**摘要**：临床研究显示，血清中重组S100A12蛋白水平与肠道炎症程度正相关，提出其作为克罗恩病和溃疡性结肠炎的无创诊断标志物。
(注：以上内容为示例性概括，实际文献需根据具体研究补充。)
S100A12. also known as EN-RAGE or calgranulin C, is a calcium-binding protein belonging to the S100 family, which comprises over 20 members involved in inflammation, cell differentiation, and immune responses. This 12 kDa protein is encoded by the *S100A12* gene and is primarily expressed in neutrophils, monocytes, and macrophages. It functions as a damage-associated molecular pattern (DAMP) molecule, playing a critical role in innate immunity by binding to receptors like RAGE (receptor for advanced glycation end products) and TLR4 (Toll-like receptor 4), thereby activating pro-inflammatory signaling pathways.
Structurally, S100A12 contains two EF-hand calcium-binding motifs, enabling calcium-dependent conformational changes that facilitate interactions with target proteins. In its recombinant form, S100A12 is produced using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. Recombinant S100A12 is widely used to study its role in chronic inflammatory diseases, including atherosclerosis, diabetes, rheumatoid arthritis, and inflammatory bowel disease, where its overexpression correlates with disease severity.
As a biomarker, S100A12 is investigated for diagnostic and prognostic value in conditions like sepsis and cancer. Its recombinant version also aids in drug discovery, enabling screening of inhibitors targeting S100A12-RAGE interactions. Despite its pathogenic roles, S100A12 exhibits antimicrobial properties, highlighting its dual function in host defense and inflammation. Ongoing research focuses on understanding its pleiotropic effects and therapeutic potential in modulating inflammatory disorders.
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