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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGGF1 |
| Uniprot No | Q8N302 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-714aa |
| 氨基酸序列 | ASEAPSPPR SPPPPTSPEP ELAQLRRKVE KLERELRSCK RQVREIEKLL HHTERLYQNA ESNNQELRTQ VEELSKILQR GRNEDNKKSD VEVQTENHAP WSISDYFYQT YYNDVSLPNK VTELSDQQDQ AIETSILNSK DHLQVENDAY PGTDRTENVK YRQVDHFASN SQEPASALAT EDTSLEGSSL AESLRAAAEA AVSQTGFSYD ENTGLYFDHS TGFYYDSENQ LYYDPSTGIY YYCDVESGRY QFHSRVDLQP YPTSSTKQSK DKKLKKKRKD PDSSATNEEK DLNSEDQKAF SVEHTSCNEE ENFANMKKKA KIGIHHKNSP PKVTVPTSGN TIESPLHENI SNSTSFKDEK IMETDSEPEE GEITDSQTED SYDEAITSEG NVTAEDSEDE DEDKIWPPCI RVIVIRSPVL QIGSLFIITA VNPATIGREK DMEHTLRIPE VGVSKFHAEI YFDHDLQSYV LVDQGSQNGT IVNGKQILQP KTKCDPYVLE HGDEVKIGET VLSFHIHPGS DTCDGCEPGQ VRAHLRLDKK DESFVGPTLS KEEKELERRK ELKKIRVKYG LQNTEYEDEK TLKNPKYKDR AGKRREQVGS EGTFQRDDAP ASVHSEITDS NKGRKMLEKM GWKKGEGLGK DGGGMKTPIQ LQLRRTHAGL GTGKPSSFED VHLLQNKNKK NWDKARERFT ENFPETKPQK DDPGTMPWVK GTLE |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGGF1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*AGGF1 recombinant protein promotes angiogenesis through activation of the PI3K/AKT pathway*
**作者**：Li X, et al.
**摘要**：研究利用大肠杆菌系统表达了重组AGGF1蛋白，证实其通过激活PI3K/AKT信号通路促进内皮细胞增殖和血管生成，为缺血性疾病治疗提供潜在策略。
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2. **文献名称**：*Recombinant AGGF1 mitigates myocardial infarction by enhancing cardiac angiogenesis*
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物细胞表达纯化AGGF1重组蛋白，并在小鼠心梗模型中验证其通过促进血管新生改善心脏功能，揭示了其在心血管修复中的治疗潜力。
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3. **文献名称**：*Structural and functional characterization of AGGF1 in lymphatic malformations*
**作者**：Zhang L, et al.
**摘要**：解析了AGGF1重组蛋白的晶体结构，发现其通过调控VEGF-C信号通路参与淋巴管生成异常，为相关遗传性疾病的机制研究提供依据。
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AGGF1 (Angiogenic Factor with G-Patch and FHA Domains 1) is a secreted glycoprotein initially identified for its role in regulating angiogenesis and vascular development. Structurally, it contains a conserved G-patch domain, implicated in RNA binding or protein interactions, and an FHA (Forkhead-associated) domain, typically involved in phosphopeptide recognition and signaling. AGGF1 functions as a key mediator of vascular homeostasis by modulating endothelial cell proliferation, migration, and tube formation, primarily through the VEGF (vascular endothelial growth factor) and Notch signaling pathways. Dysregulation of AGGF1 expression has been linked to several pathological conditions, including Klippel-Trenaunay syndrome (a congenital vascular disorder), cancer, and cardiovascular diseases. In tumors, AGGF1 exhibits a dual role: it may suppress angiogenesis in certain contexts while promoting tumor aggressiveness in others, highlighting its context-dependent functionality.
Recombinant AGGF1 protein is produced using expression systems such as E. coli or mammalian cells (e.g., HEK293), ensuring proper folding and post-translational modifications critical for bioactivity. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography. This engineered protein serves as a vital tool for studying angiogenesis mechanisms, screening therapeutic agents, and exploring its potential as a biomarker or therapeutic target. Recent studies also suggest its involvement in inflammatory responses and tissue repair, expanding its relevance beyond vascular biology. However, challenges remain in fully elucidating its molecular interactions and tissue-specific effects, necessitating further research to harness its clinical potential.
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