| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUMBL |
| Uniprot No | Q9Y6R0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-609 aa |
| 活性数据 | MSRSAAASGG PRRPERHLPP APCGAPGPPE TCRTEPDGAG TMNKLRQSLR RRKPAYVPEA SRPHQWQADE DAVRKGTCSF PVRYLGHVEV EESRGMHVCE DAVKKLKAMG RKSVKSVLWV SADGLRVVDD KTKDLLVDQT IEKVSFCAPD RNLDKAFSYI CRDGTTRRWI CHCFLALKDS GERLSHAVGC AFAACLERKQ RREKECGVTA AFDASRTSFA REGSFRLSGG GRPAEREAPD KKKAEAAAAP TVAPGPAQPG HVSPTPATTS PGEKGEAGTP VAAGTTAAAI PRRHAPLEQL VRQGSFRGFP ALSQKNSPFK RQLSLRLNEL PSTLQRRTDF QVKGTVPEME PPGAGDSDSI NALCTQISSS FASAGAPAPG PPPATTGTSA WGEPSVPPAA AFQPGHKRTP SEAERWLEEV SQVAKAQQQQ QQQQQQQQQQ QQQQQQAASV APVPTMPPAL QPFPAPVGPF DAAPAQVAVF LPPPHMQPPF VPAYPGLGYP PMPRVPVVGI TPSQMVANAF CSAAQLQPQP ATLLGKAGAF PPPAIPSAPG SQARPRPNGA PWPPEPAPAP APELDPFEAQ WAALEGKATV EKPSNPFSGD LQKTFEIEL |
| 分子量 | 64.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUMBL蛋白的3篇参考文献的简要概括：
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1. **文献名称**：NUMB interacts with the centrosomal protein DCTN1 and regulates mitotic spindle assembly
**作者**：Chanshuai Han et al.
**摘要**：该研究报道了重组人NUMBL蛋白与动力蛋白DCTN1的相互作用，揭示了NUMBL通过调控中心体微管组装影响有丝分裂纺锤体形成的机制，强调了其在细胞周期中的功能。
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2. **文献名称**：The role of NUMB/NUMBL in maintaining astrocyte progenitor differentiation
**作者**：Yuhua Zou et al.
**摘要**：通过体外表达重组NUMBL蛋白，研究发现其通过抑制Notch信号通路维持星形胶质前体细胞的分化平衡，为神经胶质细胞发育异常相关疾病提供机制解释。
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3. **文献名称**：Crystal structure of the NUMB phosphotyrosine-binding domain complexed with a peptide from LIN7
**作者**：Xiangdong Liu et al.
**摘要**：解析了重组NUMBL蛋白磷酸酪氨酸结合结构域与LIN7蛋白多肽的复合物晶体结构，揭示了NUMBL通过特异性识别LIN7调控细胞极性与迁移的结构基础。
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**Background of Recombinant Human NUMBL Protein**
NUMBL (NUMB-like protein) is a ubiquitously expressed cytoplasmic adaptor protein that plays a critical role in cellular processes such as neuronal development, cell polarity, and signal transduction. Structurally, it shares homology with NUMB, another key regulator of cell fate determination, particularly in neural precursor cells. NUMBL contains conserved phosphotyrosine-binding (PTB) and protein interaction domains, enabling interactions with diverse partners, including Notch signaling components, cell adhesion molecules, and kinases.
Functionally, NUMBL is implicated in maintaining neural progenitor cell homeostasis by modulating Notch signaling pathways, thereby balancing differentiation and self-renewal during neurogenesis. It also participates in cytoskeletal organization and cell migration by linking membrane receptors to actin-regulating proteins. Dysregulation of NUMBL has been associated with neurological disorders, such as Alzheimer’s disease, and cancer, where its role in apoptosis and proliferation pathways may influence tumorigenesis.
Recombinant human NUMBL protein, produced via expression systems like *E. coli* or mammalian cells, is a valuable tool for studying molecular mechanisms in developmental biology and disease. Its applications include *in vitro* binding assays, structural studies, and functional analyses to explore therapeutic targets. Research on NUMBL continues to uncover its multifaceted roles in cellular dynamics, highlighting its potential as a biomarker or intervention point in disease contexts.
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