| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT8 |
| Uniprot No | Q8WV74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140 aa |
| 活性数据 | MLPDCLSAEGELRCRRLLAGATARLRARPASAAVLVPLCSVRGVPALLYTLRSSRLTGRHKGDVSFPGGKCDPADQDVVHTALRETREELGLAVPEEHVWGLLRPVYDPQKATVVPVLAGVGPLDPQSLRPNSEEVSWGI |
| 分子量 | 41.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于NUDT8蛋白的研究相对较少，以下列举的参考文献为模拟示例，实际文献需通过专业数据库检索确认：
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1. **文献名称**：Characterization of human NUDT8 as a GDP-mannose hydrolase in nucleotide metabolism
**作者**：Smith A, et al.
**摘要**：该研究首次重组表达了人源NUDT8蛋白，发现其对GDP-mannose等糖核苷酸具有水解活性，提示其可能在糖代谢或信号传导中发挥作用。
2. **文献名称**：Structural insights into the catalytic mechanism of NUDT8 protein
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析了NUDT8的三维结构，揭示了其NUDIX结构域的保守催化位点，并验证了Mg²⁺依赖的底物水解机制。
3. **文献名称**：NUDT8 regulates oxidative stress response via modulating ADP-ribose levels
**作者**：Tanaka K, et al.
**摘要**：研究表明，重组NUDT8通过水解ADP-ribose调控细胞内氧化应激反应，沉默NUDT8导致细胞对H₂O₂敏感度升高。
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**注意事项**：
- NUDT8的研究文献较少，以上内容结合NUDIX家族蛋白共性模拟生成。
- 建议通过 **PubMed** 或 **UniProt(ID: Q8WVN9)** 检索最新文献，或扩展至NUDT15/NUDT1等同家族蛋白的研究作为参考。
Nudix hydrolase 8 (NUDT8), a member of the NUDIX enzyme family, is a conserved eukaryotic protein involved in nucleotide metabolism and cellular stress responses. It belongs to the NUDIX superfamily, characterized by a conserved catalytic motif (GX₅EX₇REUXEEXGU, where U represents hydrophobic residues) that enables hydrolysis of nucleoside diphosphate derivatives linked to other moieties (X). NUDT8 specifically hydrolyzes diadenosine polyphosphates (ApnA, n=3-6), signaling molecules accumulating under oxidative stress, suggesting its role in mitigating nucleotide imbalance or stress-induced toxicity. Structurally, it contains a mitochondrial targeting sequence, indicating potential localization to mitochondria, though subcellular distribution may vary across tissues.
Recombinant human NUDT8 is produced via heterologous __expression (e.g., in *E. coli* or mammalian systems) for biochemical and functional studies. Its enzymatic activity is Mg²⁺/Mn²⁺-dependent, with optimal pH around 8.0–9.0. Research links NUDT8 to redox regulation, apoptosis modulation, and mitochondrial function, though its precise physiological roles remain less characterized compared to other NUDIX members (e.g., NUDT1 or NUDT15). Dysregulation of ApnA metabolism by NUDT8 may influence pathways relevant to neurodegeneration, aging, or cancer, but mechanistic insights are limited. Current studies focus on elucidating its substrate specificity, interaction partners, and potential therapeutic applications in diseases linked to nucleotide stress or metabolic dysfunction.
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