| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT22 |
| Uniprot No | Q9BRQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303 aa |
| 活性数据 | MDPEVTLLLQ CPGGGLPQEQ IQAELSPAHD RRPLPGGDEA ITAIWETRLK AQPWLFDAPK FRLHSATLAP IGSRGPQLLL RLGLTSYRDF LGTNWSSSAA WLRQQGATDW GDTQAYLADP LGVGAALATA DDFLVFLRRS RQVAEAPGLV DVPGGHPEPQ ALCPGGSPQH QDLAGQLVVH ELFSSVLQEI CDEVNLPLLT LSQPLLLGIA RNETSAGRAS AEFYVQCSLT SEQVRKHYLS GGPEAHESTG IFFVETQNVQ RLLETEMWAE LCPSAKGAII LYNRVQGSPT GAALGSPALL PPL |
| 分子量 | 32.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUDT22蛋白的参考文献示例(注：内容为虚构，实际文献需自行检索验证)：
1. **"Functional characterization of human NUDT22 as a nucleotide phosphatase"**
*作者：Zhang L, et al. (2019)*
*摘要*：研究首次成功在大肠杆菌中重组表达并纯化人源NUDT22蛋白，鉴定其对8-oxo-dGDP等氧化损伤核苷酸的水解活性，提示其在DNA修复中的潜在作用。
2. **"Structural insights into the substrate specificity of NUDT22 through X-ray crystallography"**
*作者：Tanaka K, et al. (2020)*
*摘要*：通过X射线晶体学解析了NUDT22蛋白的三维结构，揭示其底物结合口袋的关键氨基酸残基，为开发特异性抑制剂提供结构基础。
3. **"NUDT22 expression correlates with chemoresistance in colorectal cancer"**
*作者：Wang Y, et al. (2021)*
*摘要*：发现NUDT22在结直肠癌中高表达，重组蛋白实验表明其通过降解5-氟尿嘧啶代谢产物降低药物敏感性，提示其作为治疗靶点的可能性。
4. **"Enzymatic profiling of the human NUDIX hydrolase family: Comparative analysis of NUDT22 activity"**
*作者：Smith J, et al. (2018)*
*摘要*：系统比较NUDT家族成员的酶学特性，发现重组NUDT22对dCTP和dTTP具有选择性水解能力，可能与核苷酸代谢调控相关。
建议通过PubMed或Web of Science以“NUDT22”或“NUDIX hydrolase 22”为关键词检索最新文献获取真实数据。
**Background of Recombinant Human NUDT22 Protein**
NUDT22. a member of the NUDIX hydrolase superfamily, is a conserved enzyme encoded by the *NUDT22* gene located on human chromosome Xp11.23. NUDIX proteins are characterized by a signature motif (GX₅EX₇REUXEEXGU) that facilitates hydrolysis of nucleotide substrates. While the precise biological role of NUDT22 remains under investigation, it is predicted to function in nucleotide metabolism, particularly in cleaving modified or oxidized nucleoside diphosphates to maintain cellular homeostasis. This enzymatic activity may protect against genomic instability caused by aberrant nucleotides.
Recombinant human NUDT22 protein is generated via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Structural analyses reveal a conserved NUDIX fold, with substrate specificity likely dictated by residues in its active site. Studies suggest NUDT22 may hydrolyze substrates such as 8-oxo-dGDP, linking it to oxidative damage repair pathways. Dysregulation of NUDT22 has been tentatively associated with cancers and neurodegenerative disorders, though mechanistic insights are limited.
Current research focuses on clarifying its substrate range, regulatory mechanisms, and potential therapeutic relevance. Recombinant NUDT22 serves as a critical tool for *in vitro* assays, inhibitor screening, and structural studies to unravel its role in cellular physiology and disease. Further exploration may reveal novel pathways in nucleotide surveillance or targets for drug development.
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