| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDCD3 |
| Uniprot No | Q8IVD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361 aa |
| 活性数据 | METGAAELYD QALLGILQHV GNVQDFLRVL FGFLYRKTDF YRLLRHPSDR MGFPPGAAQA LVLQVFKTFD HMARQDDEKR RQELEEKIRR KEEEEAKTVS AAAAEKEPVP VPVQEIEIDS TTELDGHQEV EKVQPPGPVK EMAHGSQEAE APGAVAGAAE VPREPPILPR IQEQFQKNPD SYNGAVRENY TWSQDYTDLE VRVPVPKHVV KGKQVSVALS SSSIRVAMLE ENGERVLMEG KLTHKINTES SLWSLEPGKC VLVNLSKVGE YWWNAILEGE EPIDIDKINK ERSMATVDEE EQAVLDRLTF DYHQKLQGKP QSHELKVHEM LKKGWDAEGS PFRGQRFDPA MFNISPGAVQ F |
| 分子量 | 40.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人NUDCD3蛋白的模拟参考文献(注：具体文献需通过PubMed或SciFinder等数据库查询确认)：
1. **"NUDCD3 promotes leukemogenesis through regulation of SMAD5 ubiquitination"**
*作者: Li X, et al.*
摘要：研究发现NUDCD3在急性髓系白血病中高表达，其通过与SMAD5相互作用促进其泛素化降解，从而激活异常造血信号通路。重组人NUDCD3蛋白的体外实验证实其促进白血病细胞增殖。
2. **"NUDCD3 is essential for hematopoietic stem cell development in zebrafish embryos"**
*作者: Wang Y, et al.*
摘要：通过斑马鱼模型揭示NUDCD3在胚胎造血干细胞分化中的关键作用，重组人NUDCD3蛋白可部分挽救NUDCD3缺失导致的造血缺陷，表明其在进化中的功能保守性。
3. **"Recombinant NUDCD3 as a potential biomarker in ovarian cancer progression"**
*作者: Chen L, et al.*
摘要：发现卵巢癌患者血清中NUDCD3水平显著升高，重组蛋白的体外功能实验显示其通过调控CDC20促进癌细胞侵袭，提示其作为治疗靶点的潜力。
注：以上内容为模拟生成的摘要，真实文献需通过学术数据库检索关键词“NUDCD3 protein”“recombinant NUDCD3”进行验证。
NudC domain-containing protein 3 (NUDCD3) is a conserved eukaryotic protein implicated in critical cellular processes, particularly nuclear movement and protein trafficking. It belongs to the NudC family, which shares homology with Aspergillus nidulans NudC, a regulator of dynein-mediated organelle transport. Human NUDCD3 is encoded by the NUDCD3 gene located on chromosome 7p22.3 and is ubiquitously expressed across tissues. Structurally, it contains a conserved NudC domain that mediates interactions with molecular motors and cytoskeletal components.
Functionally, NUDCD3 plays dual roles in nuclear translocation and post-transcriptional regulation. It assists in the nuclear import of uridine monophosphate synthetase (UMPS), a key enzyme in pyrimidine biosynthesis, through direct binding. Additionally, it stabilizes STAT5 mRNA by interacting with AU-rich elements in its 3'-UTR, modulating JAK-STAT signaling pathways crucial for hematopoiesis and immune responses.
Clinically, NUDCD3 dysregulation is linked to human diseases. Biallelic mutations cause severe combined immunodeficiency (SCID) by disrupting UMPS nuclear localization, impairing lymphocyte development. In cancers, particularly acute lymphoblastic leukemia (ALL), its overexpression correlates with poor prognosis and chemoresistance, likely through STAT5-mediated survival signals. Recent studies also suggest involvement in viral restriction pathways. These multifaceted roles position NUDCD3 as a potential therapeutic target for immune disorders and hematologic malignancies. Research continues to elucidate its precise molecular mechanisms and broader pathophysiological significance.
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