| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAT3 |
| Uniprot No | P46379-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1126aa |
| 氨基酸序列 | MEPNDSTSTAVEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASV SIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTHLPSGASS GTGSASATHGGGSPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVH INMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGPQPQHSQPPPQP PAVTPEPVALSSQTSEPVESEAPPREPMEAEEVEERAPAQNPELTPGPAP AGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAAT TDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACTPPRHLH VVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGTRPPPTPNAEAPPPG PGQASSVAPSSTNVESSAEGAPPPGPAPPPATSHPRVIRISHQSVEPVVM MHMNIQDSGTQPGGVPSAPTGPLGPPGHGQTLGQQVPGFPTAPTRVVIAR PTPPQARPSHPGGPPVSGTLQGAGLGTNASLAQMVSGLVGQLLMQPVLVA QGTPGMAPPPAPATASASAGTTNTATTAGPAPGGPAQPPPTPQPSMADLQ FSQLLGNLLGPAGPGAGGSGVASPTITVAMPGVPAFLQGMTDFLQATQTA PPPPPPPPPPPPAPEQQTMPPPGSPSGGAGSPGGLGLESLSPEFFTSVVQ GVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLS LLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPSNIRM ATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVLHC TDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGV NPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQPLPEEPME VQGAERASPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASAE TEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQG EGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDI QKRLQEDPNYSPQRFPNAQRAFADDP |
| 预测分子量 | 145 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于BAT3重组蛋白的相关文献摘要信息(注：文献标题及作者为虚拟示例，实际文献需通过学术数据库检索确认)：
1. **《BAT3重组蛋白在细胞凋亡中的调控机制研究》**
作者：Zhang L, et al.
摘要：本研究通过大肠杆菌表达系统成功制备了人源BAT3重组蛋白，发现其通过调控Bcl-2家族蛋白活性抑制线粒体途径细胞凋亡，揭示了BAT3在癌症治疗中的潜在应用价值。
2. **《重组BAT3蛋白与内质网应激的相互作用》**
作者：Kim S, et al.
摘要：文章报道了利用哺乳动物细胞表达体系纯化的BAT3重组蛋白可特异性结合未折叠蛋白，通过促进错误折叠蛋白的泛素化降解缓解内质网应激，为神经退行性疾病研究提供新方向。
3. **《BAT3重组蛋白在T细胞活化中的免疫调节功能》**
作者：Wang Y, et al.
摘要：研究证明BAT3重组蛋白通过增强T细胞受体信号通路中关键激酶的磷酸化水平，显著促进CD8+ T细胞增殖与细胞因子分泌，提示其在免疫治疗中的潜在用途。
建议通过PubMed或Web of Science搜索关键词"recombinant BAT3/BAG6 protein"获取真实文献数据。
**Background of BAT3 Recombinant Protein**
BAT3 (HLA-B-associated transcript 3), also known as BAG6 or Scythe, is a multifunctional protein encoded by the *BAG6* gene in humans. It belongs to the BAG (Bcl-2-associated athanogene) protein family, which is involved in regulating apoptosis, protein quality control, and stress responses. BAT3 plays critical roles in diverse cellular processes, including endoplasmic reticulum-associated degradation (ERAD), DNA damage repair, and immune regulation. Structurally, it contains an N-terminal ubiquitin-like domain, a central proline-rich region, and a C-terminal BAG domain, enabling interactions with chaperones, ubiquitin-proteasome components, and other signaling molecules.
Recombinant BAT3 protein is produced via genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification to study its biochemical and functional properties. Its recombinant form is widely used to investigate molecular mechanisms in protein homeostasis, apoptosis, and cancer biology. For instance, BAT3 regulates p53-dependent apoptosis by modulating its stability and activity, making it a potential therapeutic target in cancers. Additionally, it participates in MHC class I antigen presentation, linking it to immune surveillance and autoimmune diseases.
In neurodegenerative research, BAT3’s role in mitigating protein aggregation (e.g., in Alzheimer’s or Parkinson’s disease) has garnered interest. Its recombinant variants are also tools for studying post-translational modifications, such as phosphorylation, which fine-tune its interactions with client proteins. BAT3’s dual role as a chaperone and signaling adaptor underscores its importance in cellular stress adaptation, highlighting its relevance in both basic research and drug development.
Overall, BAT3 recombinant protein serves as a vital reagent for unraveling its pleiotropic functions and exploring therapeutic strategies in diseases linked to protein misfolding, apoptosis dysregulation, and immune dysfunction.
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