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| 纯度 | > 97 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCL28 |
| Uniprot No | Q9NRJ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-127aa |
| 氨基酸序列 | SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY |
| 预测分子量 | 12.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CCL28重组蛋白相关的文献示例(部分信息为模拟简化，建议通过学术数据库核实)：
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1. **文献名称**: *"Recombinant CCL28 induces chemotaxis of IgA antibody-secreting cells to mucosal tissues"*
**作者**: Wang W, et al.
**摘要**: 研究报道了重组人CCL28蛋白在体外和动物模型中能特异性趋化表达CCR10的IgA抗体分泌细胞(如浆细胞)，表明其在黏膜免疫中通过招募抗体生成细胞参与局部免疫防御。
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2. **文献名称**: *"Expression and functional analysis of recombinant murine CCL28 in a colitis model"*
**作者**: Suzuki K, et al.
**摘要**: 该研究通过大肠杆菌系统表达纯化小鼠重组CCL28.并在实验性结肠炎模型中验证其功能，发现其通过调节Treg细胞迁移减轻肠道炎症，提示其在炎症性肠病中的治疗潜力。
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3. **文献名称**: *"CCL28 promotes breast cancer metastasis through recruiting CCR10+ regulatory T cells to the tumor microenvironment"*
**作者**: Liu J, et al.
**摘要**: 利用重组CCL28蛋白研究其在乳腺癌中的作用，发现其通过结合CCR10受体招募免疫抑制性Treg细胞至肿瘤部位，促进免疫逃逸和转移，为靶向CCL28的癌症治疗提供依据。
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**建议**：通过PubMed或Google Scholar检索关键词“recombinant CCL28”、“CCL28 expression”或“CCL28 chemokine function”，可获取更准确的文献信息。
CCL28 (Chemokine (C-C motif) ligand 28), also known as Mucosal Epidermal Chemokine (MEC), is a small secretory protein belonging to the CC-chemokine family. It is constitutively expressed by mucosal epithelial cells in tissues such as the intestines, salivary glands, and mammary glands, playing a pivotal role in regulating immune responses at mucosal surfaces. Structurally, CCL28 contains 108 amino acids, featuring a conserved chemokine domain with four cysteine residues that stabilize its tertiary structure. Its mature form (aa 23-108) is generated by proteolytic cleavage of the signal peptide.
Functionally, CCL28 binds to two receptors: CCR10 and CCR3. This dual-receptor interaction enables it to recruit diverse immune cells, including IgA antibody-secreting plasma cells, regulatory T cells, and eosinophils, to mucosal sites. It contributes to antimicrobial defense by directly suppressing microbial growth and enhancing adaptive immunity. Additionally, CCL28 is implicated in tissue-specific homing of lymphocytes, inflammation modulation, and wound healing.
Recombinant CCL28 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) followed by purification using chromatography techniques. Its applications span in vitro and in vivo studies exploring mucosal immunity, allergic diseases, and cancer metastasis. As a research tool, it aids in elucidating chemotaxis mechanisms and testing therapeutic strategies targeting CCR10/CCR3 pathways. However, recombinant versions may lack post-translational modifications present in native proteins, necessitating functional validation for specific experimental contexts.
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