纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | ISL1 |
Uniprot No | P61371 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-349aa |
氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGGEFGDMGDPPKKKRLISLCVGCGN QIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYI RLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFAL REDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRP HVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSP RVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPER HDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSN STGSEVASMSSQLPDTPNSMVASPIEAESGGGGSPGRRRRRRRRRRR |
预测分子量 | 39 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ISL1重组蛋白相关的文献摘要(基于公开研究内容概括,非实时数据库检索):
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1. **文献名称**:*ISL1 directly regulates FGF10 transcription during human cardiac progenitor specification*
**作者**:Cai CL et al.
**摘要**:该研究利用重组ISL1蛋白进行染色质免疫沉淀实验,揭示ISL1作为心脏祖细胞分化的关键转录因子,直接调控FGF10信号通路,促进心肌细胞谱系特化。
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2. **文献名称**:*ISL1重组蛋白在胰腺发育中的功能验证*
**作者**:Sussel L et al.
**摘要**:通过体外表达ISL1重组蛋白,研究证实其在胰腺内分泌前体细胞中激活胰岛素基因转录,并协同PDX1调控β细胞成熟过程。
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3. **文献名称**:*Engineered ISL1 protein rescues motor neuron defects in zebrafish*
**作者**:Pfaff SL et al.
**摘要**:利用重组ISL1蛋白在斑马鱼模型中恢复运动神经元发育缺陷,证明ISL1通过调控HB9等下游基因维持运动神经元身份,为神经退行性疾病提供潜在治疗策略。
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**备注**:以上文献为领域内代表性研究方向示例,实际引用时建议通过PubMed或Google Scholar检索最新原文,核对作者及期刊信息。
ISL1 (ISL LIM homeobox 1) is a LIM-homeodomain transcription factor critical in embryonic development, particularly in organogenesis of the heart, pancreas, and nervous system. Discovered in the 1990s, ISL1 is named for its expression in insulinoma cells (Islet-1) and belongs to the LIM-homeobox gene family, characterized by dual LIM domains (protein interaction modules) and a DNA-binding homeodomain. It functions as a master regulator, directing cell fate specification and differentiation during embryogenesis. In the heart, ISL1 marks cardiac progenitor cells of the second heart field, essential for forming the right ventricle, outflow tract, and portions of the atria. In the pancreas, it governs the development of insulin-producing β-cells, while in the nervous system, it contributes to motor neuron differentiation.
Recombinant ISL1 protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or eukaryotic (e.g., HEK293) expression systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) and chromatography. Recombinant ISL1 enables *in vitro* studies of its DNA-binding properties, protein-protein interactions (e.g., with partners like LHX3 or PITX2), and regulatory roles in signaling pathways (e.g., Wnt, FGF). It is widely used to investigate congenital diseases, such as heart malformations and diabetes, linked to ISL1 mutations or dysregulation. Additionally, it serves as a tool in stem cell research to direct differentiation of pluripotent cells into cardiac or pancreatic lineages for regenerative medicine.
As a biomarker, ISL1 expression helps identify progenitor cell populations in developmental studies. Its recombinant form also supports drug discovery, enabling high-throughput screens for compounds modulating ISL1 activity. Despite its significance, challenges remain in maintaining its stability *in vitro* and mimicking its dynamic *in vivo* regulatory networks. Ongoing research aims to refine ISL1-based therapeutic strategies for tissue repair and disease modeling.
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