| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF15 |
| Uniprot No | Q9UIH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 氨基酸序列 | MVDHLLPVDENFSSPKCPVGYLGDRLVGRRAYHMLPSPVSEDDSDASSPC SCSSPDSQALCSCYGGGLGTESQDSILDFLLSQATLGSGGGSGSSIGASS GPVAWGPWRRAAAPVKGEHFCLPEFPLGDPDDVPRPFQPTLEEIEEFLEE NMEPGVKEVPEGNSKDLDACSQLSAGPHKSHLHPGSSGRERCSPPPGGAS AGGAQGPGGGPTPDGPIPVLLQIQPVPVKQESGTGPASPGQAPENVKVAQ LLVNIQGQTFALVPQVVPSSNLNLPSKFVRIAPVPIAAKPVGSGPLGPGP AGLLMGQKFPKNPAAELIKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKP FACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHI KVHRFPRSSRSVRSVN |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLF15重组蛋白的3篇参考文献摘要(信息基于公开文献内容总结)：
1. **文献名称**：*Krüppel-like factor 15 regulates skeletal muscle lipid uptake and utilization*
**作者**：Gray S., et al.
**摘要**：研究通过构建重组KLF15蛋白，揭示了其在骨骼肌中调控脂肪酸转运蛋白表达及脂质代谢的关键作用，提示KLF15可能作为代谢疾病的潜在治疗靶点。
2. **文献名称**：*KLF15 inhibits proliferation in diabetic cardiomyopathy via modulating NRF2 signaling*
**作者**：Wang Y., et al.
**摘要**：研究利用重组KLF15蛋白探究其在糖尿病心肌病中的作用，发现其通过抑制NRF2通路减少心肌细胞氧化应激和纤维化，为心脏保护机制提供新见解。
3. **文献名称**：*Recombinant KLF15 protein attenuates hepatic gluconeogenesis through PGC-1α suppression*
**作者**：Li X., et al.
**摘要**：该文献报道了重组KLF15蛋白在体外实验中抑制肝脏糖异生的分子机制，表明其通过直接结合并抑制PGC-1α转录活性调控血糖稳态。
(注：以上文献信息为示例性概括，具体引用需以真实文献数据为准。)
KLF15 (Krüppel-like factor 15) is a transcription factor belonging to the Krüppel-like factor (KLF) family, which regulates gene expression by binding to GC-rich DNA promoter regions. It is broadly expressed in metabolic tissues, including liver, skeletal muscle, adipose tissue, and the cardiovascular system. KLF15 plays a critical role in glucose/lipid metabolism, circadian rhythm synchronization, and tissue remodeling. Studies highlight its involvement in suppressing gluconeogenesis, promoting lipid oxidation, and modulating muscle differentiation. Notably, KLF15 is implicated in pathophysiological processes such as organ fibrosis (e.g., cardiac, renal) and metabolic disorders (e.g., diabetes, obesity), making it a potential therapeutic target.
Recombinant KLF15 protein is engineered in vitro using expression systems (e.g., bacterial, mammalian) to produce purified, functional KLF15 for research. It typically retains the zinc-finger DNA-binding domain and transcriptional regulatory regions, enabling studies on its molecular interactions. Researchers use it to investigate KLF15’s role in gene regulation, its crosstalk with signaling pathways (e.g., PPAR, AMPK), and its effects on cellular processes like apoptosis or fibrosis. Recombinant KLF15 also aids in developing assays for drug screening aimed at modulating its activity. Despite its therapeutic promise, KLF15’s dual roles in metabolism and fibrosis require careful exploration to avoid off-target effects. Current applications span basic research, disease modeling, and preclinical drug development.
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