| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF5 |
| Uniprot No | Q13887 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-457aa |
| 氨基酸序列 | MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLF5重组蛋白的3篇参考文献的简要信息(注：文献标题和作者为虚构示例，仅供参考格式)：
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1. **文献名称**：*KLF5 Recombinant Protein Promotes Intestinal Stem Cell Proliferation via Wnt/β-catenin Signaling*
**作者**：Zhang J, et al.
**摘要**：研究通过体外表达KLF5重组蛋白，证明其通过激活Wnt/β-catenin通路增强肠道干细胞的增殖能力，为肠道再生机制提供新见解。
2. **文献名称**：*Phosphorylation-Dependent Regulation of KLF5 Recombinant Protein in Cancer Cell Invasion*
**作者**：Suzuki H, et al.
**摘要**：探讨KLF5重组蛋白的磷酸化修饰对其功能的影响，发现磷酸化KLF5促进癌细胞侵袭，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*KLF5 Recombinant Protein Modulates Cardiac Fibroblast Activation in Myocardial Injury*
**作者**：Yang X, et al.
**摘要**：利用重组KLF5蛋白处理心肌损伤模型，发现其通过抑制TGF-β信号通路减轻心脏纤维化，为心脏修复提供实验依据。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“KLF5 recombinant protein”获取近年研究。
**Background of KLF5 Recombinant Protein**
Krüppel-like factor 5 (KLF5), a member of the Krüppel-like transcription factor family, plays a pivotal role in regulating cellular processes such as proliferation, differentiation, apoptosis, and stemness. It binds to GC-rich DNA sequences via its C-terminal zinc finger domains, modulating the expression of target genes involved in cell cycle progression (e.g., *CCND1*), survival, and oncogenic signaling. KLF5 exhibits context-dependent functions, acting as both an oncogene and tumor suppressor depending on tissue type, disease stage, and interacting partners. Its dysregulation is implicated in cancers (e.g., breast, colorectal), cardiovascular diseases, and metabolic disorders.
Recombinant KLF5 protein is engineered using expression systems like *E. coli* or mammalian cells (e.g., HEK293) to ensure proper post-translational modifications. The purified protein retains DNA-binding activity and is utilized for *in vitro* studies, including chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSA), and functional analyses of KLF5-regulated pathways. Researchers also employ it to investigate KLF5’s interplay with signaling cascades (e.g., Wnt/β-catenin, TGF-β) and its role in epithelial-mesenchymal transition (EMT) or tissue repair.
Current studies focus on KLF5’s therapeutic potential, exploring inhibitors (e.g., small molecules, CRISPR-based tools) to target its oncogenic activity. However, challenges remain in understanding its isoform-specific functions and tissue-specific regulatory networks. Recombinant KLF5 serves as a critical tool for elucidating its dual roles in disease and regeneration, paving the way for precision medicine strategies.
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