| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIMS1 |
| Uniprot No | P48059 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325aa |
| 氨基酸序列 | MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLF YEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLC QEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFK NDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPI EGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGD VCFHCNRVIEGDVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVC KKCYEKFPLELKKRLKKLAETLGRK |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LIMS1重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*PINCH1 regulates cell-matrix and cell-cell adhesions via interactions with multiple ligand molecules*
**作者**：Fukuda, T., Chen, K., Shi, X., & Wu, C.
**摘要**：该研究通过表达重组LIMS1(PINCH1)蛋白，揭示了其通过LIM结构域与整合素连接激酶(ILK)和生长因子受体结合蛋白(Grb2)的相互作用，证实了其在调控细胞黏附和信号转导中的枢纽作用。
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2. **文献名称**：*Structural and functional analysis of LIM domain-dependent recruitment of PINCH1 to focal adhesions*
**作者**：Tu, Y., Huang, Y., Zhang, Y., & Wu, C.
**摘要**：研究利用重组LIMS1蛋白进行体外结合实验和结构分析，发现其LIM结构域特异性介导与ILK的结合，并阐明了这一相互作用对细胞迁移和胞外基质组装的关键影响。
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3. **文献名称**：*Recombinant PINCH1 inhibits apoptosis in renal tubular epithelial cells via integrin-linked kinase signaling*
**作者**：Guo, L., Sanders, P. W., & Dai, C.
**摘要**：通过纯化重组LIMS1蛋白，本文证明其通过激活ILK/Akt信号通路保护肾小管上皮细胞免受凋亡，提示其在肾脏疾病中的潜在治疗价值。
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**备注**：以上文献信息为模拟示例，实际引用时建议通过PubMed或专业数据库核对原文准确性。若需具体文献，可进一步提供研究方向或时间范围。
LIMS1 (LIM and SH3 domain protein 1), also known as PINCH1. is a cytoskeletal adaptor protein that plays a critical role in cell adhesion, migration, and signal transduction. It belongs to the LIM protein family, characterized by the presence of LIM domains—zinc-binding motifs that mediate protein-protein interactions. LIMS1 contains five LIM domains and a C-terminal SH3 domain, enabling it to serve as a scaffold for assembling multiprotein complexes. It interacts with integrins, focal adhesion kinases (FAK), and other components of the integrin-linked kinase (ILK) complex, bridging the extracellular matrix (ECM) with intracellular signaling pathways.
Recombinant LIMS1 protein is engineered to study its structural and functional roles in vitro or in cellular models. Produced using expression systems like Escherichia coli or mammalian cells, the recombinant protein retains key domains for binding partners, allowing researchers to investigate its involvement in cell-ECM communication, mechanotransduction, and tissue homeostasis. Its dysregulation has been linked to pathological conditions, including cancer progression, cardiovascular diseases, and fibrosis. For instance, LIMS1 overexpression in tumors correlates with enhanced cell survival, invasion, and metastasis, making it a potential therapeutic target or biomarker.
The recombinant form is typically purified via affinity chromatography and validated for activity using assays like co-immunoprecipitation or cell adhesion studies. Its application spans basic research on cell signaling, drug discovery for targeting adhesion-related diseases, and diagnostic tool development. By providing a controlled source of functional LIMS1. recombinant protein technology accelerates mechanistic studies and therapeutic exploration, addressing both physiological and pathological contexts.
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