| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | N4BP2L2 |
| Uniprot No | Q92802 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-583 aa |
| 活性数据 | MSYGEIEGKF LGPREEVTSE PRCKKLKSTT ESYVFHNHSN ADFHRIQEKT GNDWVPVTII DVRGHSYLQE NKIKTTDLHR PLHDEMPGNR PDVIESIDSQ VLQEARPPLV SADDEIYSTS KAFIGPIYKP PEKKKRNEGR NEAHVLNGIN DRGGQKEKQK FNSEKSEIDN ELFQFYKEIE ELEKEKDGFE NSCKESEPSQ EQFVPFYEGH NNGLLKPDEE KKDLSNKAMP SHCDYQQNLG NEPDKYPCNG QVIPTFCDTS FTSFRPEWQS VYPFIVPYGP PLPSLNYHLN IQRFSGPPNP PSNIFQAQDD SQIQNGYYVN NCHVNWNCMT FDQNNEYTDC SENRSSVHPS GNGCSMQDRY VSNGFCEVRE RCWKDHCMDK HNGTDRFVNQ QFQEEKLNKL QKLLILLRGL PGSGKTTLSR ILLGQNRDGI VFSTDDYFHH QDGYRYNVNQ LGDAHDWNQN RAKQAIDQGR SPVIIDNTNI QAWEMKPYVE VAIGKGYRVE FHEPETWWKF DPEELEKRNK HGVSRKKIAQ MLDRYEYQMS ISIVMNSVEP SHKSTQRPPP PQGRQRWGGS LGSHNRVCVT NNH |
| 分子量 | 67.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人N4BP2L2蛋白的模拟参考文献示例(请注意，内容为虚构演示，实际文献需通过学术数据库查询)：
1. **文献名称**: *N4BP2L2 inhibits colorectal cancer progression by promoting ubiquitination of c-Myc*
**作者**: Zhang, L., et al.
**摘要**: 研究通过重组人N4BP2L2蛋白体外实验，发现其作为E3泛素连接酶适配体，促进致癌蛋白c-Myc的泛素化降解，抑制结直肠癌细胞增殖。
2. **文献名称**: *Structural and functional analysis of the RNA-binding domain of N4BP2L2 in neuronal development*
**作者**: Tanaka, S., et al.
**摘要**: 利用重组人N4BP2L2蛋白的RNA结合结构域进行晶体结构解析，揭示其通过调控mRNA稳定性参与神经元突触形成，缺陷导致小鼠模型神经发育异常。
3. **文献名称**: *N4BP2L2 negatively regulates antiviral signaling by targeting MAVS for proteasomal degradation*
**作者**: Wang, Y., et al.
**摘要**: 研究证明重组人N4BP2L2蛋白与线粒体抗病毒信号蛋白(MAVS)相互作用，通过泛素-蛋白酶体通路抑制RLR信号过度激活，平衡宿主抗病毒反应。
4. **文献名称**: *High-yield production of recombinant human N4BP2L2 in E. coli and its biochemical characterization*
**作者**: Müller, R., et al.
**摘要**: 开发了大肠杆菌表达系统高效制备重组人N4BP2L2蛋白，通过优化纯化条件获得高纯度蛋白，并验证其体外泛素连接酶活性及热稳定性。
**备注**：以上为示例性内容，真实文献需通过PubMed、Web of Science等平台以“N4BP2L2”、“recombinant protein”等关键词检索。
**Background of Recombinant Human N4BP2L2 Protein**
N4BP2L2 (NEDD4 Binding Protein 2-Like 2), also known as HECW2. is a ubiquitin-protein ligase belonging to the HECT family. It contains critical functional domains, including a C2 domain for calcium-dependent membrane binding, WW domains for protein-protein interactions, and a HECT domain responsible for ubiquitin transfer. N4BP2L2 regulates substrate ubiquitination, influencing protein degradation, cellular signaling, and homeostasis.
The protein plays roles in neurodevelopment, tumor suppression, and immune regulation. Studies link N4BP2L2 dysregulation to neurological disorders (e.g., autism spectrum disorders) and cancers (e.g., colorectal and lung cancers), where it may act as a tumor suppressor by targeting oncoproteins for proteasomal degradation. Its interaction with NEDD4 family E3 ligases further modulates pathways like PI3K/AKT and NF-κB.
Recombinant N4BP2L2 is typically expressed in *E. coli* or mammalian systems, purified via affinity chromatography, and used in structural studies (e.g., crystallography) or functional assays (e.g., ubiquitination activity). Ongoing research explores its therapeutic potential in cancer and neurodegenerative diseases.
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