关键信息
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基因名
manA
- 应用
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别名
manA；PMI1；Mannose-6-phosphate isomerase
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00946
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表达区间
1-391aa
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氨基酸序列
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL
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分子量
58.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The manipulation and study of recombinant proteins have become a cornerstone of modern molecular biology and biotechnology, with particular emphasis on the manA gene, which encodes for mannose-6-phosphate isomerase (MPI). This enzyme plays a critical role in the mannose metabolism pathway, catalyzing the conversion of mannose-6-phosphate to fructose-6-phosphate, and is vital for proper glycoprotein synthesis. Research on manA recombinant proteins aims to better understand the enzyme's structure, function, and interaction with other cellular components. These studies are essential for elucidating the biological significance of mannose metabolism in various organisms and its implications in human diseases, including metabolic disorders and cancer. Additionally, recombinant manA proteins offer potential applications in therapeutic development, including the engineering of glycoproteins for improved pharmacological properties. Furthermore, advances in recombinant DNA technology facilitate the expression and purification of manA proteins in various host systems, providing an invaluable tool for biochemical and pharmacological studies. As a result, research on manA recombinant proteins not only enhances our fundamental understanding of enzymatic processes but also opens avenues for innovative medical treatments, highlighting the importance of this area of study in both basic and applied sciences.
