| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MZF1 |
| Uniprot No | P28698 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-734 aa |
| 活性数据 | MRPAVLGSPD RAPPEDEGPV MVKLEDSEEE GEAALWDPGP EAARLRFRCF RYEEATGPQE ALAQLRELCR QWLRPEVRSK EQMLELLVLE QFLGALPPEI QARVQGQRPG SPEEAAALVD GLRREPGGPR RWVTVQVQGQ EVLSEKMEPS SFQPLPETEP PTPEPGPKTP PRTMQESPLG LQVKEESEVT EDSDFLESGP LAATQESVPT LLPEEAQRCG TVLDQIFPHS KTGPEGPSWR EHPRALWHEE AGGIFSPGFA LQLGSISAGP GSVSPHLHVP WDLGMAGLSG QIQSPSREGG FAHALLLPSD LRSEQDPTDE DPCRGVGPAL ITTRWRSPRG RSRGRPSTGG GVVRGGRCDV CGKVFSQRSN LLRHQKIHTG ERPFVCSECG RSFSRSSHLL RHQLTHTEER PFVCGDCGQG FVRSARLEEH RRVHTGEQPF RCAECGQSFR QRSNLLQHQR IHGDPPGPGA KPPAPPGAPE PPGPFPCSEC RESFARRAVL LEHQAVHTGD KSFGCVECGE RFGRRSVLLQ HRRVHSGERP FACAECGQSF RQRSNLTQHR RIHTGERPFA CAECGKAFRQ RPTLTQHLRV HTGEKPFACP ECGQRFSQRL KLTRHQRTHT GEKPYHCGEC GLGFTQVSRL TEHQRIHTGE RPFACPECGQ SFRQHANLTQ HRRIHTGERP YACPECGKAF RQRPTLTQHL RTHRREKPFA CQDCGRRFHQ STKLIQHQRV HSAE |
| 分子量 | 82.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MZF1蛋白的3篇参考文献示例(内容为虚构，仅供示例)：
1. **文献名称**：*"Recombinant Human MZF1 Protein Regulates Hematopoietic Stem Cell Differentiation via Target Gene Promoters"*
**作者**：Chen L, et al.
**摘要**：本研究通过在大肠杆菌系统中表达并纯化重组人MZF1蛋白，揭示了其在体外通过结合CD34等造血相关基因的启动子区域调控造血干细胞分化的分子机制。
2. **文献名称**：*"Structural Characterization of MZF1 Zinc Finger Domains Using X-ray Crystallography"*
**作者**：Wang Y, et al.
**摘要**：该研究解析了重组人MZF1蛋白的锌指结构域的晶体结构，发现其第2和第3锌指基序通过与DNA双链的磷酸骨架特异性结合，决定MZF1的靶向DNA序列选择性。
3. **文献名称**：*"Recombinant MZF1 Suppresses Tumor Growth by Inhibiting MYC Transcription in Colorectal Cancer Cells"*
**作者**：Martinez R, et al.
**摘要**：利用哺乳动物细胞表达系统获得重组人MZF1蛋白，实验表明其通过抑制癌基因MYC的转录活性，显著降低结直肠癌细胞的增殖和迁移能力。
4. **文献名称**：*"MZF1-Dependent Epigenetic Regulation of Apoptosis Pathways in Leukemia"*
**作者**：Tanaka K, et al.
**摘要**：通过重组MZF1蛋白与染色质免疫沉淀(ChIP)结合分析，发现其直接调控BCL-2家族基因的启动子甲基化水平，影响白血病细胞凋亡通路。
(注：以上文献信息为虚构，仅作示例用途。)
Recombinant human Myeloid Zinc Finger 1 (MZF1) protein is a transcription factor belonging to the zinc finger protein family, characterized by its DNA-binding zinc finger motifs. It is encoded by the *MZF1* gene, located on chromosome 19q13.4 in humans. MZF1 contains two distinct zinc finger domains: an N-terminal domain with four zinc fingers and a C-terminal domain with eight zinc fingers, each enabling sequence-specific interactions with DNA. This dual-domain structure allows MZF1 to regulate diverse biological processes, including hematopoiesis, cell differentiation, and apoptosis. Initially identified in hematopoietic cells, MZF1 is now known to be expressed in various tissues, playing roles in both promoting and repressing gene expression depending on cellular context.
Recombinant MZF1 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its involvement in cancer has drawn significant attention, as MZF1 exhibits dual roles—acting as an oncogene in leukemia, colorectal cancer, and gliomas by enhancing cell proliferation, while functioning as a tumor suppressor in breast and prostate cancers. Additionally, MZF1 regulates immune responses and tissue repair by modulating inflammatory cytokines and matrix remodeling genes. Despite these insights, its precise molecular mechanisms and context-dependent functions remain under investigation. Recombinant MZF1 serves as a critical tool for elucidating its DNA-binding specificity, protein interactions, and therapeutic potential in oncology and regenerative medicine.
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