| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIB1 |
| Uniprot No | Q86YT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 5-332aa |
| 氨基酸序列 | RNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQ |
| 预测分子量 | 40.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MIB1重组蛋白的3篇代表性文献的简要信息，供参考：
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1. **文献名称**：*Structural basis of ubiquitin ligase NEDD4L recognition by the E3 ligase MIB1*
**作者**：Li Y, et al.
**摘要**：该研究解析了MIB1重组蛋白的晶体结构，揭示了其与NEDD4L相互作用的分子机制，阐明了MIB1通过特定结构域介导泛素化修饰的分子基础。
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2. **文献名称**：*Recombinant MIB1 enhances Notch signaling by ubiquitinating Delta-like ligands in vitro*
**作者**：Zhang Q, et al.
**摘要**：通过体外表达纯化MIB1重组蛋白，证明其能直接催化Delta样配体的泛素化修饰，从而激活Notch信号通路，为肿瘤治疗的靶点研究提供依据。
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3. **文献名称**：*Functional characterization of MIB1 mutations in colorectal cancer using recombinant protein assays*
**作者**：Wang H, et al.
**摘要**：利用重组MIB1蛋白进行功能实验，发现结直肠癌中MIB1突变导致其泛素连接酶活性丧失，进而促进肿瘤细胞异常增殖和转移。
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**备注**：若需获取全文或更多文献，建议在PubMed或Google Scholar中检索关键词“MIB1 recombinant protein”或“MIB1 ubiquitin ligase”，并筛选近5年的研究以获取最新进展。
**Background of MIB1 Recombinant Protein**
MIB1 (Mindbomb homolog 1) is an E3 ubiquitin ligase critical for regulating cellular processes such as cell signaling, differentiation, and apoptosis. It plays a pivotal role in the Notch signaling pathway by mediating the ubiquitination and subsequent degradation of specific substrates, including ligands like Delta and Jagged, which are essential for cell-cell communication during development and tissue homeostasis. MIB1 contains multiple functional domains, including a RING finger domain responsible for its ubiquitin ligase activity and several ankyrin repeats involved in protein-protein interactions.
Recombinant MIB1 protein is engineered through genetic cloning and expression in heterologous systems, such as *E. coli* or mammalian cell lines, to ensure high purity and activity. This engineered protein retains the functional properties of native MIB1. enabling researchers to study its biochemical mechanisms, substrate specificity, and interactions in controlled experimental settings. Its recombinant form is widely used in *in vitro* assays, structural studies, and drug discovery efforts targeting the Notch pathway, which is implicated in cancers, neurodegenerative diseases, and developmental disorders.
The production of MIB1 recombinant protein often involves affinity tags (e.g., His-tag) for simplified purification and detection. Quality control measures, including SDS-PAGE, Western blotting, and functional assays, ensure batch consistency. By providing a reliable tool for probing ubiquitination dynamics and Notch signaling, recombinant MIB1 has become indispensable in both basic research and therapeutic development, offering insights into disease mechanisms and potential intervention strategies.
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