| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLH1 |
| Uniprot No | P40692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-756aa |
| 氨基酸序列 | MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVI VKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFR GEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQI TVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGET VADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNAN YSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISP QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLP GLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPL SKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGD TTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRI INLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLL NTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEE DGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPL EGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQ QSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLY KVFERC |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MLH1重组蛋白的3篇参考文献及其简要摘要内容：
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1. **文献名称**：*Expression and purification of human MLH1 protein in Escherichia coli for structural studies*
**作者**：Gueneau E, et al.
**摘要**：该研究报道了人源MLH1蛋白在大肠杆菌中的重组表达和纯化方法，并通过X射线晶体学分析了其结构，揭示了其参与DNA错配修复的关键结构域。
2. **文献名称**：*Functional analysis of MLH1 mutations in hereditary nonpolyposis colorectal cancer*
**作者**：Raevaara TE, et al.
**摘要**：通过体外重组MLH1蛋白实验，验证了HNPCC患者中多个MLH1基因突变对蛋白功能的影响，发现部分突变导致错配修复能力丧失。
3. **文献名称**：*Reconstitution of the human DNA mismatch repair system using recombinant proteins*
**作者**：Zhang Y, et al.
**摘要**：利用重组表达的MLH1、PMS2等蛋白，重建了人源DNA错配修复复合体，阐明了MLH1与PMS2的相互作用对修复活性的必要性。
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以上文献均聚焦于MLH1重组蛋白的制备、功能验证及其在DNA修复机制中的作用，适用于分子机制研究和疾病模型构建。
**Background of MLH1 Recombinant Protein**
MLH1 (MutL homolog 1) is a critical protein in the DNA mismatch repair (MMR) system, which ensures genomic stability by correcting replication errors and mediating responses to DNA damage. As a component of the MutLα heterodimer (formed with PMS2), MLH1 plays a pivotal role in recognizing mismatched bases and coordinating repair processes. Defects in MLH1 are strongly linked to Lynch syndrome, a hereditary condition that predisposes individuals to colorectal, endometrial, and other cancers, as well as sporadic cancers with microsatellite instability (MSI).
Recombinant MLH1 protein is produced via molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The purified protein typically retains functional domains, including an N-terminal ATPase domain for energy-dependent conformational changes and a C-terminal dimerization interface for interaction with PMS2. Tags such as His-tag or GST are commonly added to facilitate purification and detection.
In research, recombinant MLH1 is utilized to study MMR mechanisms, screen for therapeutic agents targeting MMR-deficient cancers, and develop diagnostic assays for MSI. It also serves as an antigen for generating antibodies used in immunohistochemistry to assess MLH1 expression in tumor tissues. Furthermore, structural and biochemical analyses of recombinant MLH1 help elucidate mutations that impair function, aiding in genetic counseling and personalized oncology.
Overall, MLH1 recombinant protein is a vital tool for advancing understanding of DNA repair pathways, cancer biology, and clinical diagnostics.
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