| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIB2 |
| Uniprot No | Q96AX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-955aa |
| 氨基酸序列 | MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于MIB2重组蛋白的参考文献示例(文献内容为模拟概括，仅供参考)：
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1. **文献名称**: *Structural and Functional Analysis of MIB2 Reveals a Conserved Mechanism of E3 Ubiquitin Ligase Activation*
**作者**: Smith J, Doe R, Lee T.
**摘要**: 本研究解析了人源MIB2重组蛋白的晶体结构，揭示了其E3泛素连接酶活性依赖的RING结构域构象变化，并通过体外泛素化实验证明MIB2对Notch信号通路配体DLL1的泛素化修饰作用。
2. **文献名称**: *MIB2-Mediated Ubiquitination Regulates RIP1-Dependent Apoptosis in Inflammatory Signaling*
**作者**: Zhang Y, Wang F, Chen L.
**摘要**: 通过重组MIB2蛋白的体外实验，研究发现MIB2通过泛素化修饰RIP1激酶，调控细胞凋亡和NF-κB信号通路，揭示了其在炎症反应中的新功能。
3. **文献名称**: *Engineering and Characterization of Recombinant MIB2 for Targeted Protein Degradation Studies*
**作者**: Kumar S, Patel A, Nguyen H.
**摘要**: 报道了利用大肠杆菌表达系统高效纯化重组MIB2蛋白的方法(含His标签)，并验证其与E2泛素结合酶UBE2D1的协同作用，为蛋白降解靶向嵌合体(PROTAC)设计提供工具。
4. **文献名称**: *MIB2 E3 Ligase Activity Requires Dimeric Conformation: Insights from In Vitro Reconstitution*
**作者**: Garcia M, Kim E, Rossi M.
**摘要**: 通过重组MIB2蛋白的体外二聚化实验，证明其E3连接酶活性依赖自身二聚体形成，并发现特定突变体(如Cys-to-Ala突变)可显著抑制泛素化功能。
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**备注**：以上文献为示例，实际引用需根据具体研究领域补充真实文献(可检索PubMed/Google Scholar关键词：*MIB2 recombinant protein, E3 ligase, ubiquitination*)。建议结合最新研究(如2020年后)或经典高引论文。
MIB2 (Mind Bomb 2) is an E3 ubiquitin ligase that plays a critical role in regulating ubiquitination-dependent signaling pathways, particularly the Notch signaling cascade. As a member of the MIB protein family, it facilitates the transfer of ubiquitin molecules to specific substrate proteins, marking them for proteasomal degradation or functional modulation. MIB2 is structurally characterized by multiple functional domains, including a MIB-specific N-terminal domain, tandem zinc-finger motifs, and a C-terminal RING finger domain essential for its E3 ligase activity. These domains enable MIB2 to interact with substrates like the Notch receptor ligands (e.g., Delta, Jagged) and mediate their ubiquitination, thereby influencing cell-cell communication, cell fate determination, and developmental processes.
Recombinant MIB2 proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional MIB2 for experimental studies. These proteins retain key biochemical properties, allowing researchers to investigate MIB2’s enzymatic mechanisms, substrate specificity, and regulatory roles in vitro or in cellular models. MIB2’s involvement in critical pathways has linked it to diseases, including cancers and neurological disorders, where dysregulated ubiquitination contributes to pathogenesis. For instance, aberrant MIB2 expression may disrupt Notch signaling, affecting tissue homeostasis. Recombinant MIB2 thus serves as a tool for drug discovery, enabling high-throughput screening for inhibitors or activators to restore pathway balance. Its study also sheds light on the broader ubiquitin-proteasome system, advancing therapeutic strategies targeting E3 ligases.
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