纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MRPL41 |
Uniprot No | Q8IXM3 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 14-137 aa |
活性数据 | GADRMSK WTSKRGPRSF RGRKGRGAKG IGFLTSGWRF VQIKEMVPEF VVPDLTGFKL KPYVSYLAPE SEETPLTAAQ LFSEAVAPAI EKDFKDGTFD PDNLEKYGFE PTQEGKLFQL YPRNFLR |
分子量 | 15.3 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL41蛋白的模拟参考文献示例(基于现有领域知识合理概括,非真实文献):
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1. **《MRPL41 induces mitochondrial apoptosis via caspase-dependent pathway》**
- **作者**: Zhang Y, et al.
- **摘要**: 本研究通过原核系统成功表达并纯化了重组人MRPL41蛋白,发现其在HeLa细胞中过表达可激活caspase-3/9.促进线粒体膜电位下降及细胞凋亡,表明MRPL41可能通过线粒体凋亡通路调控程序性细胞死亡。
2. **《Structural insights into MRPL41-p53 interaction in mitochondrial ribosome assembly》**
- **作者**: Li H, et al.
- **摘要**: 利用重组MRPL41蛋白的晶体结构解析,揭示其与肿瘤抑制因子p53的相互作用界面,表明MRPL41可能通过结合p53参与线粒体核糖体组装及DNA损伤应激响应。
3. **《Downregulation of MRPL41 in hepatocellular carcinoma correlates with poor prognosis》**
- **作者**: Wang X, et al.
- **摘要**: 通过肝癌组织样本分析,发现MRPL41表达显著下调,且重组MRPL41蛋白可抑制肝癌细胞增殖并诱导G1期阻滞,提示其作为潜在抑癌分子在肝癌治疗中的作用。
4. **《Epigenetic regulation of MRPL41 by promoter methylation in colorectal cancer》**
- **作者**: Kim SJ, et al.
- **摘要**: 研究证实结直肠癌中MRPL41启动子区高甲基化导致其表达沉默,重组MRPL41蛋白回补实验可恢复线粒体功能并抑制肿瘤生长,为表观遗传靶向治疗提供依据。
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注:以上内容为领域知识合理推演,具体文献需通过PubMed、Google Scholar等平台以“MRPL41”或“mitochondrial ribosomal protein L41”为关键词检索确认。
**Recombinant Human MRPL41 Protein Overview**
Mitochondrial Ribosomal Protein L41 (MRPL41) is a nuclear-encoded component of the mitochondrial large ribosomal subunit, essential for mitochondrial translation and oxidative phosphorylation. It plays a critical role in assembling the respiratory chain complexes required for ATP production. Beyond its ribosomal function, MRPL41 is implicated in apoptosis regulation. Studies suggest it activates caspase-dependent apoptotic pathways by stabilizing p53 and modulating Bcl-2 family proteins, linking mitochondrial metabolism to cell survival decisions.
Dysregulation of MRPL41 is associated with cancer. It acts as a tumor suppressor in certain contexts, where its downregulation correlates with poor prognosis and enhanced tumor growth. Conversely, elevated MRPL41 may promote apoptosis in stressed cells, highlighting its context-dependent roles. Recombinant human MRPL41 protein, produced via bacterial or eukaryotic expression systems, enables functional studies of its dual roles in mitochondrial biology and apoptosis. Its applications include elucidating molecular mechanisms of diseases linked to mitochondrial dysfunction (e.g., cancers, neurodegenerative disorders) and screening therapeutic agents targeting metabolic or apoptotic pathways. Structural analysis of recombinant MRPL41 also aids in understanding ribosome assembly and protein-protein interactions within mitochondria.
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