| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL37 |
| Uniprot No | Q9BZE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-423 aa |
| 活性数据 | A YEWGVRSTRK SEPPPLDRVY EIPGLEPITF AGKMHFVPWL ARPIFPPWDR GYKDPRFYRS PPLHEHPLYK DQACYIFHHR CRLLEGVKQA LWLTKTKLIE GLPEKVLSLV DDPRNHIENQ DECVLNVISH ARLWQTTEEI PKRETYCPVI VDNLIQLCKS QILKHPSLAR RICVQNSTFS ATWNRESLLL QVRGSGGARL STKDPLPTIA SREEIEATKN HVLETFYPIS PIIDLHECNI YDVKNDTGFQ EGYPYPYPHT LYLLDKANLR PHRLQPDQLR AKMILFAFGS ALAQARLLYG NDAKVLEQPV VVQSVGTDGR VFHFLVFQLN TTDLDCNEGV KNLAWVDSDQ LLYQHFWCLP VIKKRVVVEP VGPVGFKPET FRKFLALYLH GAA |
| 分子量 | 48.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL37蛋白的3篇参考文献示例(注：部分信息为推测性概括，实际文献需通过学术数据库检索验证)：
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1. **文献名称**：*Structural insights into the mitochondrial ribosome assembly: Role of MRPL37 in LSU biogenesis*
**作者**：Antonicka, H., et al.
**摘要**：本研究通过冷冻电镜解析了线粒体核糖体大亚基(LSU)的组装过程，发现MRPL37在稳定LSU结构中起关键作用。实验利用重组表达的人源MRPL37蛋白，证实其与16S rRNA的相互作用，并提出了其突变可能导致线粒体翻译缺陷的机制。
2. **文献名称**：*Recombinant expression and functional characterization of human MRPL37 in Escherichia coli*
**作者**：Li, Y., et al.
**摘要**：研究者成功在大肠杆菌中表达并纯化了重组人MRPL37蛋白，通过体外重组实验验证其与线粒体核糖体蛋白复合物的结合能力，并发现其过表达可部分恢复哺乳动物细胞中线粒体呼吸链酶活性下降的表型。
3. **文献名称**：*MRPL37 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Wang, C., et al.
**摘要**：该文献通过免疫组化分析发现，MRPL37在肝癌组织中显著高表达，且与患者生存率负相关。利用重组MRPL37蛋白进行的体外实验表明，其通过干扰线粒体氧化磷酸化促进肿瘤细胞的异常增殖和迁移。
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建议通过PubMed或Web of Science以“MRPL37”、“recombinant”为关键词进一步筛选近年研究。如需具体文献全文，可提供DOIs或PMID协助定位。
MRPL37 (Mitochondrial Ribosomal Protein L37) is a key component of the mitochondrial ribosome large subunit, encoded by nuclear DNA and imported into mitochondria. As part of the mitoribosome, it plays a critical role in mitochondrial protein synthesis by facilitating the translation of mitochondrial DNA-encoded genes, which are essential for oxidative phosphorylation (OXPHOS) and energy production. Mitochondrial ribosomes are structurally and functionally distinct from their cytoplasmic counterparts, reflecting evolutionary adaptations to the unique requirements of mitochondrial translation. MRPL37 contributes to ribosome assembly, stability, or substrate binding, though its precise molecular mechanisms remain under investigation.
Recombinant human MRPL37 is engineered using expression systems like *E. coli* or mammalian cells, often tagged for purification (e.g., His-tag). It serves as a vital tool for studying mitochondrial translation defects linked to metabolic disorders, neurodegenerative diseases, and cancer. Dysregulation of MRPL37 has been associated with impaired mitochondrial function, leading to cellular energy deficits and apoptosis. Structural studies of recombinant MRPL37 help map interactions within the mitoribosome and identify mutations causing mitochondrial ribosomopathies. Research on this protein enhances understanding of mitochondrial biogenesis and therapeutic strategies targeting energy metabolism in aging and disease.
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