| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL34 |
| Uniprot No | Q9BQ48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-92 aa |
| 活性数据 | MAVLAGSLLGPTSRSAALLGGRWLQPRAWLGFPDAWGLPTPQQARGKARGNEYQPSNIKRKNKHGWVRRLSTPAGVQVILRRMLKGRKSLSH |
| 分子量 | 36.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL34蛋白的假设参考文献示例，供参考(请注意部分内容可能为虚构，实际文献需通过学术数据库验证)：
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1. **文献名称**：**"Structural Insights into Human Mitochondrial Ribosome Assembly: The Role of MRPL34"**
**作者**：Brown, A., Amunts, A.
**摘要**：该研究利用冷冻电镜技术解析了人线粒体核糖体大亚基的结构，揭示了MRPL34在核糖体组装中的关键作用。通过重组表达MRPL34蛋白并结合突变分析，发现其通过与相邻蛋白相互作用维持核糖体稳定性，为线粒体翻译机制提供了新见解。
2. **文献名称**：**"Heterologous Expression and Functional Analysis of Recombinant Human MRPL34 in Yeast"**
**作者**：Lee, S., Kim, D.
**摘要**：研究报道了在酵母系统中重组表达人源MRPL34蛋白的方法，纯化后通过互补实验证实其可修复线粒体功能障碍表型，表明MRPL34在跨物种中具有保守的核糖体组装功能。
3. **文献名称**：**"MRPL34 Promotes Hepatocellular Carcinoma via Enhancing Mitochondrial Oxidative Phosphorylation"**
**作者**：Zhang, X., Wang, Y.
**摘要**：通过重组MRPL34蛋白的过表达实验，发现其显著增强肝癌细胞的线粒体氧化磷酸化能力，促进肿瘤增殖。临床数据分析显示MRPL34与肝癌患者不良预后相关。
4. **文献名称**：**"Mitochondrial Ribosomal Protein L34: From Structure to Human Disease"**(综述)
**作者**：Koc, E.C., Spremulli, L.L.
**摘要**：综述总结了MRPL34的基因定位、重组表达技术及其在疾病中的作用，指出其突变可能导致线粒体脑肌病等疾病，并强调其在核糖体-线粒体质量控制中的潜在调控机制。
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**注意**：以上文献名称、作者及摘要内容为示例性虚构，真实研究需查阅权威数据库(如PubMed、Web of Science)。建议结合具体研究需求进一步检索关键词“MRPL34”、“mitochondrial ribosome”、“recombinant protein”等获取准确文献。
**Background of Recombinant Human MRPL34 Protein**
The mitochondrial ribosomal protein L34 (MRPL34) is a component of the large subunit of the mitochondrial ribosome, essential for mitochondrial translation and oxidative phosphorylation (OXPHOS). Encoded by nuclear DNA, MRPL34 is imported into mitochondria, where it participates in assembling the ribosomal machinery responsible for synthesizing proteins critical to the electron transport chain. MRPL34 is evolutionarily conserved, highlighting its fundamental role in mitochondrial function. Dysregulation of MRPL34 has been linked to mitochondrial disorders, cancer, and metabolic diseases, as impaired ribosome biogenesis disrupts cellular energy production and homeostasis.
Recombinant human MRPL34 protein is produced through *in vitro* expression systems (e.g., *E. coli* or mammalian cells) using genetic engineering, enabling high-purity yields for functional studies. It serves as a vital tool to investigate MRPL34’s structure, interactions, and role in mitochondrial protein synthesis. Research applications include elucidating molecular mechanisms underlying mitochondrial pathologies, screening for therapeutic targets, and studying compensatory responses to mitochondrial stress. Additionally, recombinant MRPL34 aids in developing diagnostic assays or protein replacement strategies for mitochondrial diseases. Its study contributes to advancing precision medicine approaches for disorders tied to mitochondrial dysfunction.
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