| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL16 |
| Uniprot No | Q9NX20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-251 aa |
| 活性数据 | PSFE DVSIPEKPKL RFIERAPLVP KVRREPKNLS DIRGPSTEAT EFTEGNFAIL ALGGGYLHWG HFEMMRLTIN RSMDPKNMFA IWRVPAPFKP ITRKSVGHRM GGGKGAIDHY VTPVKAGRLV VEMGGRCEFE EVQGFLDQVA HKLPFAAKAV SRGTLEKMRK DQEERERNNQ NPWTFERIAT ANMLGIRKVL SPYDLTHKGK YWGKFYMPKR V |
| 分子量 | 28.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL16蛋白的3篇参考文献示例(注：部分文献为模拟概括，可能存在虚构或简化内容，建议通过学术数据库核实)：
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1. **标题**: *"Characterization of the human mitochondrial ribosomal protein L16 gene (MRPL16) and its functional role in oxidative phosphorylation"*
**作者**: Smith A, et al.
**摘要**: 该研究首次克隆并鉴定了人MRPL16基因，发现其编码线粒体核糖体大亚基的核心蛋白。通过重组表达实验，验证了MRPL16蛋白在维持线粒体核糖体结构完整性及促进氧化磷酸化相关mRNA翻译中的关键作用。
2. **标题**: *"Cryo-EM structure of the human mitochondrial ribosome reveals the role of MRPL16 in subunit assembly"*
**作者**: Brown K, et al.
**摘要**: 利用冷冻电镜解析人线粒体核糖体高分辨率结构，发现MRPL16定位于大亚基的界面区域，参与稳定rRNA与相邻蛋白的相互作用。通过重组蛋白突变实验，证实其缺失会导致核糖体亚基组装异常。
3. **标题**: *"MRPL16 deficiency causes mitochondrial translation defects linked to neurodegenerative disorders"*
**作者**: Chen L, et al.
**摘要**: 通过CRISPR敲除细胞模型，发现MRPL16缺陷导致线粒体编码蛋白(如复合物Ⅰ亚基)合成减少，引发ATP生成障碍。研究利用重组MRPL16蛋白进行功能回补实验，证明了其在神经退行性疾病中的潜在病理机制。
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**备注**：以上文献为示例性质，实际研究中可查阅PubMed、Google Scholar等平台，以“MRPL16”、“mitochondrial ribosome”、“recombinant MRPL16”等关键词检索最新论文。
The mitochondrial ribosomal protein L16 (MRPL16) is a key component of the large subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Encoded by nuclear DNA and imported into mitochondria, MRPL16 plays a critical role in assembling and stabilizing the ribosome structure, enabling the translation of mitochondrial DNA (mtDNA)-encoded mRNAs. These mRNAs primarily encode subunits of the oxidative phosphorylation (OXPHOS) complexes, which drive ATP production. Dysregulation of MRPL16 has been linked to mitochondrial dysfunction, contributing to diseases such as neurodegenerative disorders, metabolic syndromes, and cancer. As a recombinant protein, MRPL16 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. Research focuses on its interactions with other ribosomal proteins, rRNA, and regulatory factors, as well as its potential as a biomarker or therapeutic target. Recombinant MRPL16 also aids in modeling mitochondrial translation defects and screening compounds to restore OXPHOS activity. Its study advances understanding of mitochondrial biology and diseases rooted in energy metabolism disruptions.
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