| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL15 |
| Uniprot No | P49406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292 aa |
| 活性数据 | MAACIAAGHWAAMGLGRSFQAARTLLPPPASIACRVHAGPVRQQSTGPSEPGAFQPPPKPVIVDKHRPVEPERRFLSPEFIPRRGRTDPLKFQIERKDMLERRKVLHIPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMKPVVQEPNQKVPVNELKVKMKPKPWSKRWERPNFNIKGIRFDLCLTEQQMKEAQKWNQPWLEFDMMREYDTSKIEAAIWKEIEASKRS |
| 分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL15蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
1. **文献名称**：*Expression and Purification of Recombinant Human MRPL15 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了重组人MRPL15蛋白的原核表达系统，利用大肠杆菌进行高效表达，并通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**：*Structural Insights into Mitochondrial Ribosomal Protein MRPL15 by Cryo-EM*
**作者**：Zhang L, et al.
**摘要**：通过冷冻电镜解析了MRPL15在线粒体核糖体大亚基中的三维结构，揭示了其与rRNA的结合模式及在翻译中的潜在作用位点。
3. **文献名称**：*MRPL15 Overexpression Promotes Apoptosis Resistance in Colorectal Cancer Cells*
**作者**：Garcia R, et al.
**摘要**：研究发现MRPL15在结直肠癌细胞中异常高表达，通过调控线粒体翻译活性抑制细胞凋亡，提示其作为潜在治疗靶点。
4. **文献名称**：*Functional Interaction of MRPL15 with Mitochondrial Translation Elongation Factors*
**作者**：Kim H, et al.
**摘要**：利用重组MRPL15蛋白进行体外结合实验，证实其与延伸因子mtEF-Tu的相互作用，并探讨该互作对线粒体蛋白质合成的调控机制。
**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献，使用关键词如“recombinant MRPL15”、“mitochondrial ribosome L15”等。
**Background of Recombinant Human MRPL15 Protein**
The mitochondrial ribosomal protein L15 (MRPL15) is a key component of the mitochondrial ribosome, a specialized machinery responsible for synthesizing proteins essential for mitochondrial function. Encoded by nuclear DNA, MRPL15 is translated in the cytoplasm and subsequently imported into mitochondria, where it integrates into the large subunit (39S) of the mitochondrial ribosome (55S). Mitochondrial ribosomes differ structurally from their cytoplasmic counterparts, reflecting adaptations for translating mitochondrion-encoded mRNAs, which primarily encode subunits of the oxidative phosphorylation (OXPHOS) complexes.
MRPL15 plays a critical role in ribosome assembly and stability, facilitating proper protein synthesis within mitochondria. Dysregulation of MRPL15 has been linked to mitochondrial dysfunction, impacting cellular energy production and potentially contributing to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Recombinant human MRPL15 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, interactions, and functional roles. This recombinant tool enables investigations into mitochondrial translation mechanisms, disease pathophysiology, and therapeutic targeting. Its applications span *in vitro* assays, structural studies, and functional genomics, offering insights into mitochondrial biology and pathologies linked to ribosomal defects.
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