| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL14 |
| Uniprot No | Q6P1L8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-145 aa |
| 活性数据 | AIQKMTRVRV VDNSALGNSP YHRAPRCIHV YKKNGVGKVG DQILLAIKGQ KKKALIVGHC MPGPRMTPRF DSNNVVLIED NGNPVGTRIK TPIPTSLRKR EGEYSKVLAI AQNFV |
| 分子量 | 15.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL14蛋白的3篇参考文献摘要，格式遵循要求：
1. **文献名称**：*Mitochondrial ribosomal protein L14 contributes to cell apoptosis*
**作者**：Li Y, et al.
**摘要**：该研究通过构建重组人MRPL14蛋白，发现其过表达可激活线粒体依赖性凋亡通路，并证实其在氧化应激条件下参与调控细胞凋亡过程。
2. **文献名称**：*Structural insights into the assembly of the mitochondrial large ribosomal subunit*
**作者**：Brown A, et al.
**摘要**：利用重组表达的MRPL14蛋白进行冷冻电镜分析，揭示了其在线粒体核糖体大亚基组装中的作用，并提出其可能作为结构支架维持rRNA稳定性。
3. **文献名称**：*MRPL14 mutations impair ribosomal assembly and cause developmental disorders*
**作者**：Smith J, et al.
**摘要**：通过重组蛋白功能回补实验，证实MRPL14的基因突变会导致线粒体翻译缺陷，进而引发能量代谢异常和神经发育障碍。
注：以上文献为示例性内容，实际研究需通过学术数据库(如PubMed、Web of Science)检索确认。
MRPL14 (Mitochondrial Ribosomal Protein L14) is a nuclear-encoded component of the mitochondrial ribosome, specifically part of the 39S large subunit essential for mitochondrial protein synthesis. Mitochondria rely on their own translational machinery to produce subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP generation. MRPL14 plays a structural and functional role in maintaining ribosome integrity, facilitating the assembly and stabilization of the mitochondrial ribosome during translation of mitochondrial DNA-encoded mRNAs.
The human MRPL14 gene is located on chromosome 6 (6q21) and consists of multiple exons. Defects in MRPL14 or mitochondrial ribosomal proteins are linked to mitochondrial disorders, often presenting as metabolic, neurological, or muscular impairments due to disrupted OXPHOS activity. Recombinant MRPL14 protein, produced via heterologous expression systems like E. coli or mammalian cells, serves as a key tool for studying mitochondrial translation mechanisms, protein-protein interactions, and disease pathogenesis. Its use in functional assays, structural studies, and disease models helps elucidate molecular pathways underlying mitochondrial dysfunction, offering potential therapeutic insights. Research on MRPL14 also contributes to understanding the crosstalk between nuclear and mitochondrial genomes, highlighting its broader relevance in cellular energy homeostasis and disease.
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