| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPP1 |
| Uniprot No | Q00013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466 aa |
| 活性数据 | MTLKASEGES GGSMHTALSD LYLEHLLQKR SRPEAVSHPL NTVTEDMYTN GSPAPGSPAQ VKGQEVRKVR LIQFEKVTEE PMGITLKLNE KQSCTVARIL HGGMIHRQGS LHVGDEILEI NGTNVTNHSV DQLQKAMKET KGMISLKVIP NQQSRLPALQ MFMRAQFDYD PKKDNLIPCK EAGLKFATGD IIQIINKDDS NWWQGRVEGS SKESAGLIPS PELQEWRVAS MAQSAPSEAP SCSPFGKKKK YKDKYLAKHS SIFDQLDVVS YEEVVRLPAF KRKTLVLIGA SGVGRSHIKN ALLSQNPEKF VYPVPYTTRP PRKSEEDGKE YHFISTEEMT RNISANEFLE FGSYQGNMFG TKFETVHQIH KQNKIAILDI EPQTLKIVRT AELSPFIVFI APTDQGTQTE ALQQLQKDSE AIRSQYAHYF DLSLVNNGVD ETLKKLQEAF DQACSSPQWV PVSWVY |
| 分子量 | 52.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人MPP1蛋白的参考文献示例(文献内容为虚构，仅用于演示格式)：
1. **文献名称**: *Structural insights into the human MPP1 protein through recombinant expression and crystallography*
**作者**: Li, X.; Zhang, Y.; Wang, H.
**摘要**: 本研究报道了人源MPP1蛋白的重组表达和纯化方法(基于大肠杆菌系统)，并通过X射线晶体学解析了其PDZ结构域的三维结构，揭示了其与红细胞膜骨架蛋白相互作用的分子机制。
2. **文献名称**: *Functional characterization of recombinant MPP1 in membrane-associated guanylate kinase complexes*
**作者**: Müller, S.; Schmidt, A.B.
**摘要**: 利用杆状病毒-昆虫细胞系统重组表达MPP1.研究其在MAGUK蛋白复合体中的支架功能，发现MPP1通过SH3结构域调控细胞连接处的信号转导，并证实其缺失导致细胞极性异常。
3. **文献名称**: *Development of a high-yield purification protocol for recombinant human MPP1 and its palmitoylation analysis*
**作者**: Gomez, R.; Patel, K.
**摘要**: 开发了一种基于镍亲和层析和尺寸排阻色谱的MPP1重组蛋白高效纯化方案，并利用质谱技术证明了其翻译后棕榈酰化修饰对细胞膜定位的关键作用。
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**备注**：以上文献信息为示例，若需真实文献，建议通过PubMed或Web of Science检索关键词(如 "recombinant MPP1" "human" + "expression" "structure")，并优先选择近5年发表的生物化学或结构生物学领域论文。
Recombinant human MPP1 (Membrane Protein, Palmitoylated 1), also known as PALM or MAGUK p55 subfamily member 1. is a scaffold protein belonging to the membrane-associated guanylate kinase (MAGUK) family. It plays a critical role in organizing membrane microdomains and maintaining cell polarity, particularly in erythrocytes and epithelial cells. MPP1 contains characteristic protein interaction domains: a PDZ domain, an SH3 domain, and a guanylate kinase-like (GUK) domain, enabling it to link transmembrane proteins with cytoskeletal components and signaling molecules.
Originally identified in erythrocyte membranes, MPP1 contributes to the mechanical stability of red blood cells by interacting with the cytoskeleton through its association with protein 4.1R and glycophorin C. Beyond structural roles, MPP1 participates in signal transduction pathways and cellular processes like vesicle trafficking, synaptic formation, and cell adhesion. Its palmitoylation facilitates membrane anchoring, crucial for domain-specific localization and function.
Recombinant MPP1 is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for studying its structural, biochemical, and functional properties. Research focuses on its implications in diseases, including X-linked intellectual disability (XLID) linked to MPP1 mutations, cancer metastasis involving membrane dynamics, and blood disorders like hereditary spherocytosis. Its versatility as a modular scaffold protein makes it a valuable model for understanding membrane-cytoskeleton interactions and developing therapeutic strategies.
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