| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMEL1 |
| Uniprot No | Q495T6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-779 aa |
| 活性数据 | MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWIDEESKKKAQEKAMSIREQIGHPDYILEETNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW |
| 分子量 | 115.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MMEL1蛋白的3篇代表性文献摘要整理：
1. **文献名称**：Structural and functional analysis of human metalloendopeptidase MMEL1
**作者**：Smith J et al.
**摘要**：该研究解析了重组人MMEL1蛋白的晶体结构，发现其N端催化结构域具有锌离子依赖性蛋白酶活性，并证明其特异性水解多肽底物的能力，为神经肽调控机制提供依据。
2. **文献名称**：Recombinant expression and enzymatic characterization of MMEL1 in Alzheimer's disease
**作者**：Wang L, Kim S
**摘要**：成功在大肠杆菌中表达功能性重组MMEL1.发现其对β-淀粉样蛋白具有降解活性，提示其在阿尔茨海默病病理中的潜在作用及治疗应用前景。
3. **文献名称**：MMEL1 modulates inflammatory response through cleavage of chemokines
**作者**：Garcia-Ruiz C et al.
**摘要**：通过哺乳动物细胞表达系统获得高纯度重组MMEL1.揭示其通过裂解CXCL8/CXCL12等趋化因子调控炎症反应的功能机制，为免疫疾病研究提供新靶点。
(注：以上为虚构摘要，实际研究中建议通过PubMed等数据库检索具体文献)
MMEL1 (Membrane Metalloendopeptidase-Like 1), also known as NL1 or neprilysin-like protein 1. is a zinc-dependent metalloprotease belonging to the M13 peptidase family, which includes neprilysin (NEP) and endothelin-converting enzymes. It is a type II transmembrane or secreted glycoprotein expressed in various tissues, including the brain, kidney, and testis. MMEL1 shares structural homology with NEP, featuring a conserved catalytic domain responsible for hydrolyzing peptide bonds, but exhibits distinct substrate preferences. It is implicated in the proteolytic processing of bioactive peptides, such as amyloid-β (Aβ) peptides, opioid neuropeptides, and glucagon-like peptides, thereby influencing neurological, metabolic, and immune pathways.
Research highlights its potential role in neurodegenerative diseases, particularly Alzheimer's disease (AD), where it may contribute to Aβ clearance. In contrast to NEP, MMEL1 demonstrates preferential cleavage of oligomeric Aβ species, suggesting unique therapeutic relevance. Additionally, MMEL1 regulates pain perception by degrading encephalin and participates in fertility-related pathways. Recombinant human MMEL1 protein, typically produced in mammalian or insect expression systems with proper post-translational modifications, enables in vitro studies of its enzymatic kinetics, substrate specificity, and inhibitor screening. Its soluble extracellular domain is often purified via affinity tags for functional assays. Current investigations focus on targeting MMEL1 for modulating peptide hormone homeostasis or developing biomarkers for AD and metabolic disorders. However, its physiological regulation and pathophysiological contributions remain incompletely characterized, necessitating further mechanistic exploration.
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