| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLKLAK |
| Uniprot No | Q9NYL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-800 aa |
| 活性数据 | MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI EKEAEILSVL SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE EMDMDHIMTW ATDVAKGMHY LHMEAPVKVI HRDLKSRNVV IAADGVLKIC DFGASRFHNH TTHMSLVGTF PWMAPEVIQS LPVSETCDTY SYGVVLWEML TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH QCWEADAKKR PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRCEIEAT LERLKKLERD LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LPSFEIGAWT EDDVYCWVQQ LVRKGDSSAE MSVYASLFKE NNITGKRLLL LEEEDLKDMG IVSKGHIIHF KSAIEKLTHD YINLFHFPPL IKDSGGEPEE NEEKIVNLEL VFGFHLKPGT GPQDCKWKMY MEMDGDEIAI TYIKDVTFNT NLPDAEILKM TKPPFVMEKW IVGIAKSQTV ECTVTYESDV RTPKSTKHVH SIQWSRTKPQ DEVKAVQLAI QTLFTNSDGN PGSRSDSSAD CQWLDTLRMR QIASNTSLQR SQSNPILGSP FFSHFDGQDS YAAAVRRPQV PIKYQQITPV NQSRSSSPTQ YGLTKNFSSL HLNSRDSGFS SGNTDTSSER GRYSDRSRNK YGRGSISLNS SPRGRYSGKS QHSTPSRGRY PGKFYRVSQS ALNPHQSPDF KRSPRDLHQP NTIPGMPLHP ETDSRASEED SKVSEGGWTK VEYRKKPHRP SPAKTNKERA RGDHRGWRNF |
| 分子量 | 91.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设您所指的可能是与激酶或细胞凋亡相关的重组人蛋白(如MLKL)的文献示例。由于“MLKLAK”名称未在主流数据库中检索到，可能存在拼写误差或领域特殊性。建议核实蛋白名称后再进一步查询。
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**参考文献示例：**
1. **文献名称**：*MLKL mediates apoptosis through caspase-8 activation induced by ROS-dependent ER stress*
**作者**：Wang H, et al.
**摘要**：研究揭示了重组人MLKL蛋白通过诱导活性氧(ROS)依赖的内质网应激，激活caspase-8通路促进细胞凋亡的分子机制。
2. **文献名称**：*Recombinant human MLKL protein purification and structural characterization*
**作者**：Zhang Y, et al.
**摘要**：报道了重组人MLKL蛋白在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其激酶样结构域的三维结构。
3. **文献名称**：*The role of MLKL in necroptosis: Insights from in vitro reconstitution assays*
**作者**：Murphy JM, et al.
**摘要**：利用重组表达的MLKL蛋白，验证了其在坏死性凋亡中的核心作用，并阐明其与RIPK3的磷酸化互作机制。
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**注意**：若您所指的蛋白涉及其他功能或名称变体，请提供更多背景信息以便精准检索。
Recombinant human MLKLAK (Mixed Lineage Kinase family) proteins are engineered signaling molecules involved in regulating critical cellular processes, including stress responses, apoptosis, and inflammation. Belonging to the serine/threonine kinase family, MLKLAK kinases typically act upstream of mitogen-activated protein kinase (MAPK) pathways, such as JNK and p38 cascades, modulating gene expression and cell fate decisions. Their recombinant forms are generated via heterologous expression systems (e.g., E. coli or mammalian cells) to study kinase activity, substrate interactions, or signaling mechanisms in vitro.
Research on MLKLAK proteins has highlighted their dual roles: promoting apoptosis under oxidative stress while mediating pro-survival signals in specific contexts. Dysregulation of these kinases is implicated in neurodegenerative diseases, cancer, and autoimmune disorders, making them potential therapeutic targets. Recombinant variants enable structural studies (e.g., crystallography) to design inhibitors or activators.
Despite their significance, functional redundancy within the MLK family and context-dependent signaling pose research challenges. Recent advances in CRISPR and omics technologies aim to clarify their tissue-specific roles. The development of phospho-specific antibodies using recombinant MLKLAK proteins has further accelerated pathway mapping. Ongoing studies focus on harnessing their regulatory potential for precision medicine, particularly in inflammation and neurodegeneration.
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