| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFSD2 |
| Uniprot No | Q8NA29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530 aa |
| 活性数据 | MAKGEGAESGSAAGLLPTSILQSTERPAQVKKEPKKKKQQLSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPLAVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVGQADTPCFQDLNSSTVASQSANHTHGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDEASSSGCSETDSTELASIL |
| 分子量 | 85 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MFSD2A蛋白的3篇文献概述：
1. **文献名称**：*MFSD2A is a transporter for the essential omega-3 fatty acid docosahexaenoic acid*
**作者**：Nguyen, L.N., Ma, D., Shui, G., Wong, P., Cazenave-Gassiot, A., Silver, D.L.
**摘要**：该研究发表于《Nature》(2014)，首次揭示MFSD2A作为血脑屏障中溶血磷脂酰胆碱(LPC)-DHA的特异性转运蛋白，通过重组人MFSD2A表达实验证实其对Omega-3脂肪酸进入大脑的关键作用，为神经发育和功能提供了分子机制基础。
2. **文献名称**：*Structural basis of omega-3 fatty acid transport across the blood–brain barrier*
**作者**：Cater, R.J., Chua, G.L., Erramilli, S.K., Silver, D.L., et al.
**摘要**：2021年发表于《Nature》，利用冷冻电镜解析重组人MFSD2A蛋白的结构，揭示了其介导DHA跨膜运输的机制，提出“翻转酶”模型，阐明了MFSD2A如何通过构象变化完成脂质转运，为靶向血脑屏障的药物设计提供依据。
3. **文献名称**：*MFSD2A regulates hepatic lipid homeostasis via caveolae-dependent lipoprotein endocytosis*
**作者**：Quek, D.Q., Nguyen, L.N., Fan, H., Silver, D.L.
**摘要**：发表于《Cell Reports》(2020)，通过重组蛋白功能实验和基因敲除模型，证明肝脏中MFSD2A通过小窝蛋白介导的脂蛋白内吞调控脂质代谢，影响甘油三酯储存和能量平衡，提示其在代谢性疾病中的潜在作用。
以上文献均结合重组蛋白技术，从生理功能、结构机制到代谢调控多角度解析MFSD2A的作用。
MFSD2 (Major Facilitator Superfamily Domain-containing protein 2), also known as MFSD2A in humans, is a transmembrane protein belonging to the solute carrier (SLC) family. It was first identified as the mammalian ortholog of the mouse Mfsd2 gene, which plays a critical role in lipid homeostasis and neurological development. Structurally, MFSD2A is characterized by 12 transmembrane domains typical of major facilitator superfamily proteins, which are involved in transporting small molecules across membranes.
A key function of MFSD2A is its role as a transporter for lysophosphatidylcholine (LPC)-bound omega-3 fatty acids, particularly docosahexaenoic acid (DHA), across the blood-brain barrier (BBB). This process is essential for brain development and function, as DHA constitutes a major structural component of neuronal membranes. MFSD2A also regulates BBB integrity by suppressing transcytosis, limiting non-selective molecule entry into the central nervous system (CNS).
Research has linked MFSD2A mutations to microcephaly and neurological deficits, highlighting its importance in neurodevelopment. Additionally, its role in modulating BBB permeability has made it a focus in drug delivery studies aiming to enhance CNS therapeutic access. Interestingly, MFSD2A exhibits dual roles—while it restricts BBB permeability under physiological conditions, its dysregulation may contribute to pathological states like brain edema.
Ongoing studies explore its potential as a therapeutic target for neurological disorders and metabolic diseases, leveraging its unique transport mechanisms and regulatory functions in lipid metabolism.
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