| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKX6-1 |
| Uniprot No | P78426 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS SSSSSSSSSP SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASASSASAAA AAAAAAAAAA SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS AAAVAAVGRY PKPLAELPGR TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR PTFSGQQIFA LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS GGGGGLLLHA SEPESSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKX6-1重组蛋白的模拟参考文献示例(非真实文献，仅供参考)：
1. **文献名称**：*Recombinant NKX6-1 Protein Enhances Pancreatic β-cell Differentiation from Human Embryonic Stem Cells*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化重组NKX6-1蛋白，证明其可显著促进人多能干细胞分化为胰岛素分泌β细胞，为糖尿病细胞治疗提供了潜在工具。
2. **文献名称**：*Structural Insights into the DNA-binding Domain of NKX6-1 Transcription Factor*
**作者**：Zhang L, et al.
**摘要**：利用X射线晶体学解析了重组NKX6-1的DNA结合结构域三维结构，揭示了其与靶基因启动子结合的关键位点，为调控其活性的药物设计提供了依据。
3. **文献名称**：*Functional Characterization of Recombinant NKX6-1 in Insulin Gene Regulation*
**作者**：Wang H, et al.
**摘要**：通过体外实验证实重组NKX6-1蛋白直接结合胰岛素基因启动子，并与PDX1协同增强转录活性，阐明其在β细胞功能维持中的关键作用。
4. **文献名称**：*High-Yield Production of Recombinant NKX6-1 in Mammalian Expression Systems*
**作者**：Kimura T, et al.
**摘要**：优化哺乳动物细胞表达系统，实现重组NKX6-1的高效生产，并验证其在动物模型中促进β细胞再生的效果，支持糖尿病治疗的临床转化研究。
(注：以上为模拟生成的参考文献，实际文献需通过学术数据库查询。)
NKX6-1 recombinant protein is derived from the human NK6 homeobox 1 (NKX6-1) gene, which encodes a transcription factor belonging to the NKX family of homeodomain-containing proteins. This family plays critical roles in developmental processes, particularly in the specification and maintenance of cell lineages. NKX6-1 is predominantly expressed in pancreatic β-cells and the central nervous system (CNS), where it regulates key functions such as β-cell differentiation, insulin production, and neuronal subtype development. Its role in pancreatic islet cells has made it a focal point in diabetes research, as NKX6-1 deficiency or dysfunction is linked to impaired insulin secretion and β-cell maturation.
The recombinant form of NKX6-1 is typically produced using expression systems like *E. coli* or mammalian cell lines, ensuring high purity and bioactivity for experimental applications. It retains the functional homeodomain responsible for DNA binding and transcriptional regulation, enabling researchers to study its interaction with target genes, such as those involved in insulin biosynthesis (e.g., *INS*, *PDX1*) or neuronal patterning. NKX6-1 recombinant protein is widely used in *in vitro* studies to explore mechanisms of β-cell regeneration, diabetes therapeutics, and neurodevelopmental pathways. Additionally, it serves as a tool for investigating NKX6-1's potential role in diseases beyond diabetes, including certain cancers where its expression may be dysregulated. As a reagent, it supports drug screening, protein-protein interaction assays, and functional studies aimed at deciphering its dual roles in metabolic and neurological health.
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