| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOMO1 |
| Uniprot No | Q15155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1222aa |
| 氨基酸序列 | MLVGQGAGPLGPAVVTAAVVLLLSGVGPAHGSEDIVVGCGGFVKSDVEINYSLIEIKLYTKHGTLKYQTDCAPNNGYFMIPLYDKGDFILKIEPPLGWSFEPTTVELHVDGVSDICTKGGDINFVFTGFSVNGKVLSKGQPLGPAGVQVSLRNTGTEAKIQSTVTQPGGKFAFFKVLPGDYEILATHPTWALKEASTTVRVTNSNANAASPLIVAGYNVSGSVRSDGEPMKGVKFLLFSSLVTKEDVLGCNVSPVPGFQPQDESLVYLCYTVSREDGSFSFYSLPSGGYTVIPFYRGERITFDVAPSRLDFTVEHDSLKIEPVFHVMGFSVTGRVLNGPEGDGVPEAVVTLNNQIKVKTKADGSFRLENITTGTYTIHAQKEHLYFETVTIKIAPNTPQLADIIATGFSVCGQISIIRFPDTVKQMNKYKVVLSSQDKDKSLVTVETDAHGSFCFKAKPGTYKVQVMVPEAETRAGLTLKPQTFPLTVTNRPMMDVAFVQFLASVSGKVSCLDTCGDLLVTLQSLSRQGEKRSLQLSGKVNAMTFTFDNVLPGKYKISIMHEDWCWKNKSLEVEVLEDDMSAVEFRQTGYMLRCSLSHAITLEFYQDGNGRENVGIYNLSKGVNRFCLSKPGVYKVTPRSCHRFEQAFYTYDTSSPSILTLTAIRHHVLGTITTDKMMDVTVTIKSSIDSEPALVLGPLKSVQELRREQQLAEIEARRQEREKNGNEEGEERMTKPPVQEMVDELQGPFSYDFSYWARSGEKITVTPSSKELLFYPPSMEAVVSGESCPGKLIEIHGKAGLFLEGQIHPELEGVEIVISEKGASSPLITVFTDDKGAYSVGPLHSDLEYTVTSQKEGYVLTAVEGTIGDFKAYALAGVSFEIKAEDDQPLPGVLLSLSGGLFRSNLLTQDNGILTFSNLSPGQYYFKPMMKEFRFEPSSQMIEVQEGQNLKITITGYRTAYSCYGTVSSLNGEPEQGVAMEAVGQNDCSIYGEDTVTDEEGKFRLRGLLPGCVYHVQLKAEGNDHIERALPHHRVIEVGNNDIDDVNIIVFRQINQFDLSGNVITSSEYLPTLWVKLYKSENLDNPIQTVSLGQSLFFHFPPLLRDGENYVVLLDSTLPRSQYDYILPQVSFTAVGYHKHITLIFNPTRKLPEQDIAQGSYIALPLTLLVLLAGYNHDKLIPLLLQLTSRLQGVRALGQAASDNSGPEDAKRQAKKQKTRRT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOMO1重组蛋白的示例参考文献(注：以下文献信息为示例性虚构内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Cloning and Expression Analysis of NOMO1 Recombinant Protein in Neural Development"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过基因克隆技术成功表达NOMO1重组蛋白，并验证其在神经干细胞分化中的潜在作用，揭示了NOMO1在神经突触形成中的分子机制。
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2. **文献名称**: *"Structural Characterization of NOMO1 Protein and Its Interaction with Wnt Signaling Pathway"*
**作者**: Hiroshi K, et al.
**摘要**: 利用重组NOMO1蛋白进行晶体结构解析，发现其与Wnt通路关键蛋白β-catenin存在直接结合，提示NOMO1可能通过调控Wnt信号影响肿瘤发生。
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3. **文献名称**: *"NOMO1 Recombinant Protein as a Biomarker in Colorectal Cancer"*
**作者**: Smith R, et al.
**摘要**: 通过免疫组化分析发现，NOMO1重组蛋白在结直肠癌组织中高表达，且与患者预后不良显著相关，表明其可能作为潜在治疗靶点。
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4. **文献名称**: *"Functional Analysis of NOMO1 in Apoptosis Regulation via Recombinant Protein Overexpression"*
**作者**: Chen Y, et al.
**摘要**: 在HEK293细胞中过表达NOMO1重组蛋白，发现其通过抑制Caspase-3活性减少细胞凋亡，为NOMO1的抗凋亡机制提供了实验依据。
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**建议**：实际研究中，可通过PubMed、Google Scholar等平台以关键词"NOMO1 recombinant protein"或"NOMO1 gene function"检索最新文献，并优先选择高影响力期刊(如*Nature*、*Cell*子刊)或领域内权威论文。
**Background of NOMO1 Recombinant Protein**
The NOMO1 (Nodal Modulator 1) protein, encoded by the *NOMO1* gene, is a component of the Nodal signaling pathway, which plays a critical role in embryonic development, particularly in establishing body asymmetry and tissue patterning. NOMO1 belongs to the NOMO/PRELI family of proteins and shares structural homology with the *Drosophila* protein Boccino. It functions as a chaperone-like molecule, facilitating the transport or stability of other signaling components. Notably, NOMO1 interacts with the Nodal co-receptor Cripto-1. modulating the activity of transforming growth factor-beta (TGF-β) superfamily ligands, which regulate cellular processes like differentiation, proliferation, and apoptosis.
Recombinant NOMO1 protein is engineered using expression systems such as *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. This recombinant form retains the functional domains required for ligand-receptor interactions, making it a valuable tool for studying Nodal signaling mechanisms *in vitro*. Researchers utilize NOMO1 recombinant protein to investigate its role in developmental biology, cancer (where TGF-β pathways are often dysregulated), and regenerative medicine. Additionally, it aids in screening therapeutic agents targeting Nodal-associated pathologies, including metastatic cancers and congenital disorders linked to developmental signaling errors. Its application extends to structural studies, antibody production, and elucidating molecular interactions within the TGF-β signaling network. As a reagent with high purity and specificity, recombinant NOMO1 continues to advance both basic and translational research in developmental and disease contexts.
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